DEVELOPMENT OF A SEMI-AUTOMATED METABOLITE BATCH EXTRACTION DEVICE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 获取高质量代谢组学数据的一个关键组成部分是提取过程。提取是费时和乏味的。这一瓶颈导致生产率降低。此外，当手动处理大批量样品时，样品提取容易出错，导致样品质量和再现性下降。为了解决这些问题，我们开发了一种半自动代谢物批量提取装置。使用该设备，我们希望实现高度可重复的提取，可以在短时间内进行，高通量的方式。为了验证数据的重现性，我们将通过NMR分析手动和半自动提取方法的牛肝提取物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A key component for acquiring high quality metabolomics data is the extraction process. Extractions are time-consuming and tedious. This bottleneck leads to lower productivity. Furthermore, sample extraction is prone to error when large batches are handled manually, leading to a decrease in sample quality and reproducibility. To address these problems, we have developed a semi-automated metabolite batch extraction device. Using this device we hope to achieve highly repoducible extractions that can be performed in a short, high throughput manner. To verify data reproducibility we will analyze beef liver extracts from manual and semi-automated extraction methods by NMR.