1-D PROTON NMR OF 2 SAMPLES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 研究方法： NMR光谱 通过溶解在D2 O（99.9%D）中并冷冻干燥，将每个样品的两个20 mg等分试样进行氘交换。然后将样品溶解于0.7 mL D2 O（99.96%D）中，并置于5 mm NMR管中。在Varian Inova-800光谱仪上在25 ℃下获得1-D质子NMR光谱。 谱宽为12789.8 Hz。 对于每个样品，取8次扫描的平均值。 化学位移参考HDO信号（在25 ℃下，Δ =4.77 ppm）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: NMR Spectroscopy Two 20 mg aliquots of each sample were deuterium exchanged by dissolving in D2O (99.9 % D) and freeze-drying. The samples were then dissolved in 0.7 mL D2O (99.96 % D) and placed in a 5-mm NMR tube. 1-D Proton NMR spectra were acquired on a Varian Inova-800 spectrometer at 25 ¿C. Spectral width was 12789.8 Hz. For each sample 8 scans were averaged. Chemical shifts were referenced to the HDO signal (¿=4.77 ppm at 25 ¿C).