IMAGING INTERACTION OF STEM CELLS WITH BRAIN SLICE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的初步数据表明，干细胞可以向脑肿瘤微环境迁移。我们希望通过使用离体脑切片模型来可视化干细胞如何向脑肿瘤迁移。在这里，我们将通过共聚焦显微镜来确定干细胞在脑切片微环境中的行为。具体而言，（1）通过三维重构，观察干细胞在脑片内的迁移（向更深层次的侵入），观察迁移路径;（2）通过神经突的延伸，观察干细胞与脑片内的驻留细胞的接触;（3）研究脑片微环境对干细胞分化的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our preliminary data establish that stem cells can migrate toward brain tumor microenvironment. We would like to visualize how stem cells migrate toward the brain tumor by using ex vivo brain slice model. Here, we will determine how stem cells behave in brain slice microenviornment by using confocal microscopy. Specifically, we will (1) Examine the migration of stem cells inside the brain slice (invasion into a deeper level) by 3-D reconstitution to visualize the migratory path; (2) Examine how the stem cells contact with the brain slice residential cells by neurite extension; and (3) Examine the effects of brain slice microenvironment on the differentiation of stem cells.