APPROACHES TO IMPROVE PROTEIN FOOTPRINTING: HIGH PRESSURE DIGESTION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白酶对蛋白质的不完全消化是质谱法表征蛋白质的障碍。 有些蛋白质由于聚集而难以消化。 为了提高蛋白酶的消化效率，我们研制了一种在线高压消化系统。 该系统使用UPLC技术将消解压力提高到最大10，000 psi。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The incomplete digestion of proteins by proteases is a hindrance in their characterization by mass spectrometry. Some proteins are hard to digest due to aggregation. In order to increase the digestion efficiency of proteases we have developed an on-line high pressure digestion system. This system uses UPLC technology to increase the digestion pressure to a maximum of 10,000 psi.