LC TESTING AND PERFORMANCE OF MS INSTRUMENTATION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本项目的目的是使用Agilent LC和Eksigent系统以及ABI/Sciex、ESI QSTAR、ESI-QSTAR XL仪器优化毛细管液相色谱，以便在低飞摩尔水平下常规完成色谱分离，然后进行质谱检测和数据依赖性肽测序。 （在合作项目和其他技术研究与开发项目下报告了额外的工作量和仪器时间。）

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The purpose of this project is to optimize capillary liquid chromatography using the Agilent LC and Eksigent systems with the ABI/Sciex, ESI QSTAR, ESI-QSTAR XL instruments so that chromatographic separations followed by mass spectrometric detection and data dependent peptide sequencing can be accomplished routinely at the low femtomole level. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)