Facility Biospecimen and Xenograft Core

项目总结(见说明)： Biosecimen核心(核心B)为项目1-4提供部分基础设施支持。该核心将根据二十多年的经验，提供组织良好的标准化样本收集、存储、分发和相关临床/研究信息传播系统。组织标本的病理分析将有一致性和质量保证。Biospecimen Core还参与了Core研究员开发的一大系列前列腺癌异种移植株的维护工作。这些异种移植物用于POI调查人员提议的研究，每项研究都由核心人员监督。总体而言，该核心小组的运营分为4个组成部分： 1.标本的获取、处理、质量控制、储存和进入数据库； 2.前列腺癌异种移植的维持、开发和研究计划。 3.实验室服务，包括由泌尿科病理学家解释组织和组织定位研究、免疫组织化学(IHC)、定量RT-PCR和免疫分析，即PSA和组织培养。该中心还将利用我们的异种移植资源进行临床前研究；以及 4.从少数族裔患者那里获取样本、确定样本分配的优先顺序、实施质量控制计划以及确保患者保密和遵守IRB要求的行政计划。 本计划项目中详细说明的每个研究项目的执行情况要求研究人员能够随时获得有良好记录的临床标本和相关的生物模型。领导Biospecimen Core的研究人员也将其作为一项政策，提供过量的前列腺癌 在世界范围内向POI合作者和非P01调查人员提供标本资源。总体而言， Biosecimen Core是这一P01计划项目的关键组成部分。

PROJECT SUMMARY (See instructions): The Biospecimen Core (Core B) provides part of the infrastructure support for Projects 1 - 4. This Core will provide a well-organized and standardized system of specimen collection, storage, distribution and related clinical/research information dissemination that is based on over two decades of experience. There will be consistency and quality assurance in the pathological analysis of tissue specimens. The Biospecimen Core is also involved in the maintenance of a large series of prostate cancer xenograft lines developed by Core investigators. These xenografts are used for proposed studies by the POI investigators and each study is overseen by Core personnel. Overall, the operations of this Core group into 4 components: 1. Specimen acquisition, processing, quality control, storage and accessioning into databases; 2. A prostate cancer xenograft maintenance, development and study program. 3. Laboratory services, including interpretation of tissues and tissue localization studies by a urologic pathologist, immunohistochemistry (IHC), quantitative RT-PCR, and immunoassays, i.e, PSA, and tissue culture. The Core will also perform pre-clinical studies using our xenograft resources; and 4. An administrative program to obtain samples from minority patients, prioritize the distribution of specimens, conduct a quality control program, and to ensure patient confidentiality and compliance with IRB requirements. The performance of each of the research projects detailed in this program project mandates that investigators have ready access to well documented clinical specimens and relevant biological models. The investigators directing the Biospecimen Core also make it a policy to provide excess prostate cancer specimen resources to POI collaborators and to non-P01 investigators on a world-wide basis. Overall, the Biospecimen Core is a critical component of this P01 program project.