Transgenic Gene Knockout

通过转基因和基因敲除技术建立的动物模型在评估基因功能方面具有不可估量的价值 以及确定某些基因如何调节细胞生长和分化， 这些基因会导致癌症。小鼠在该设施中通过原核注射 将DNA注入受精卵，通过将逆转录病毒注射到受精卵的卵周间隙，或通过注射 突变的胚胎干细胞（ES细胞）转化为囊胚。这个共享资源的目的是提供 在最具时间和成本效益的情况下， 路上了此外，这些小鼠品系的可用性极大地刺激了 St Jude的调查员。

Animal models created by transgenic and gene knockout technology are invaluable in evaluating gene function in vivo and in determining how certain genes regulate cell growth and differentiation and how alterations in these genes contribute to cancer. Mice are genetically modified in this facility either by pronucleus injection of DNA into fertilized eggs, by injection of retroviruses into the perivitteline space of fertilized eggs, or by injection of mutated embryonic stem cells (ES-cells) into blastocysts. The objective of this shared resource is to provide genetically modified mice to principal Investigators within the Cancer Center in the most time- and costeffective way. In addition, the availability of these mouse strains greatly stimulates interactions among investigators at St Jude.