Gastric Histopathology Core

胃组织病理学核心的主要使命是提供高质量、快速周转的组织学 和免疫组织化学服务于此PPG的研究人员，并提供专家组织病理学 翻译，通过与凯·华盛顿博士的合作。建议提供以下服务： 1.为PPG研究人员提供高质量、快速周转的研究组织学服务。 2.为幽门螺杆菌诱导的大鼠胃肿瘤模型的组织病理学提供专家评价 并与人类疾病相关。 3.在动物免疫组织化学研究的开发、执行和评估方面提供专业知识 纸巾。 4.提供激光显微解剖服务。 5.执行质量控制，以确保组织学和免疫组织化学服务是最高的 质量。 6.提供组织芯片服务，并根据需要获取人胃组织样本。 这一拟议计划项目的三个组成部分项目和核心B(蛋白质组学核心)都依赖于 幽门螺杆菌诱导鼠模型胃组织的形态分析 胃炎和胃肿瘤。华盛顿博士在解释组织病理学方面的专业知识 啮齿动物模型的改变和免疫组织化学研究的解释将是这项工作的关键， 这项合作活动目前不是通过现有资源提供资金的。通过利用的资源 已建立的研究组织学设施，用于组织存储、加工、切片和染色 幻灯片，它将有可能达到高标准的所有组织学研究建议在这项拨款。这 在外科病理学家的监督下集中组织学服务 胃肠道病理将允许仔细注意质量保证。

The primary mission of the Gastric Histopathology Core is to provide high quality, rapid-turnaround histology and immunohistochemistry services to investigators in this PPG, and to provide expert histopathologic nterpretation, through collaboration with Dr. Kay Washington. The following services are proposed: 1. To provide high quality, rapid-turnaround research histology services to PPG investigators. 2. To provide expert evaluation of histopathology of rodent models of H. pylori-Induced gastric neoplasia and correlation with human disease. 3. To provide expertise in developing, performing and evaluating immunohistochemical studies on animal tissues. 4. To provide laser microdissection services. 5. To perform quality control to ensure that histology and immunohistochemistry services are of highest quality. 6. To provide tissue microarray services and access to human gastric tissue samples as needed. The three component projects and Core B (Proteomics Core) of this proposed Program Project all rely heavily upon morphologic analysis of gastric tissues isolated from rodent models of Helicobacter pyloriinduced gastritis and gastric neoplasia. Dr. Washington's expertise in interpretation of histopathologic changes in rodent models and in interpretation of immunohistochemical studies will be critical to this work, and this collaborative activity is currently not funded through existing resources. By utilizing the resources of an established Research Histology facility for tissue storage, processing, cutting of blocks, and staining of slides, it will be possible to achieve high standards for all histologic studies proposed in this grant. This centralization of histologic services under the supervision of a surgical pathologist with a dedicated interest in gastrointestinal pathology will allow careful attention to quality assurance.