BIOTECHNOLOGY CORE

项目总结（见说明）： CCCR的生物技术核心将设在南卡罗来纳州大学仪器资源设施（IRF）。IRF是一个成熟的生物技术核心，有20年的历史，为研究人员提供了广泛的显微镜，细胞分选，小动物成像和几种分子技术的最先进的设备。该设施配备了三名全职教师，两名兼职教师和四名技术人员，协助研究人员设计实验和赠款提案，处理研究样品，操作设备以及培训教师，员工和学生。还提供几个国家认可的讲习班和研究生课程 通过IRF。IRF的资金来自各种来源，包括NIH用于购买主要设备的赠款，联邦和私人来源的各种资助计划的教师合作，用户费用和大学支持。生物技术核心的目标将通过三个具体目标来实现：1）提供广泛的技术和主要设备，包括但不限于流式细胞术和细胞分选，组织学，免疫组织化学，活细胞和共聚焦成像，电子显微镜，以及小动物超声和荧光成像; 2）提供技术专业知识，协助和培训CCCR研究人员及其实验室成员使用生物技术核心中可用的设备; 3）通过小组会议和咨询，确定和准备获得新技术的建议，这将有利于CCCR和其他研究人员的研究计划。

PROJECT SUMMARY (See instructions): The Biotechnology Core for the CCCR will be housed in the University of South Carolina Instrumentation Resource Facility (IRF). The IRF is an established biotechnology core, with a 20 year history, that provides a wide range of state-of-the-art equipment for microscopy, cell sorting, small animal imaging, and several molecular techniques for researchers. The facility is staffed with three full time faculty, two part time faculty, and four technicians that assist investigators with design of their experiments and grant proposals, processing of research samples, operation of equipment, and training of faculty, staff and students. Several nationally recognized workshops and graduate level courses are also available through the IRF. Funding of the IRF is generated from a variety of sources including grants from NIH for acquisition of major equipment, faculty collaborations on a variety of funding programs from federal and private sources, user fees, and University support. The goals of the Biotechnology Core will be addressed through three Specific Aims: 1) to provide access to a wide range of techniques and major equipment including, but not limited to, flow cytometry and cell sorting, histology, immunohistochemistry, live cell and confocal imaging, electron microscopy, and small animal ultrasound and fluorescence imaging; 2) to provide the technical expertise to assist and train CCCR Investigators and members of their laboratories on the use of equipment available in the Biotechnology Core; and 3) through group meetings and consultation identify and prepare proposals to acquire new technology which will benefit the research programs of the CCCR and other investigators.