Multi-modal AO-LSO Phase Gradient Imaging of the Inner Retina

项目总结/摘要 强大的新型自适应光学成像技术，通过波- 前置传感器，并使用可变形反射镜波前补偿器（如自适应光学）对其进行校正 扫描激光检眼镜（AOSLO）和AO-OCT提供详细的解剖，生理和 在人眼细胞水平上的功能信息。物理科学公司(PSI)最近 展示了一种新的方法，称为自适应光学线扫描检眼镜（AO-LSO）。的 PSI发明和开发的临床AO-LSO视网膜成像仪，大大简化了AO光学设计， 消除了高速扫描组件，与研究相比减少了临床足迹 AOSLO，同时保留了（线）共焦成像用于分辨视网膜层的许多优点。 尽管AOSLO和AO-LSO提供了常规成像和计数视锥光感受器的能力， 目前，没有直接的方法用于人眼中视网膜神经节细胞的体内成像，或者 计算内层视网膜的细胞体。我们在第一阶段的目标是展示一种先进的新设计， AO-LSO成像仪（包括一个AO-OCT通道）采用了新的高灵敏度摄像技术， 线照明方法的相位梯度成像的内部视网膜。设想的平台将是 设计为紧凑的临床系统，用于灵活的体内细胞成像应用。AO-LSO 在第一阶段，将使用新的线阵相机技术对原型仪器进行修改，并用于调查 新的成像模式。如果成功，这种方法和相关的成像算法将在 II期，用于监测疾病进展/消退并表征视网膜内重塑， 波士顿儿童医院的病人临床研究者将在I期提供反馈，并将领导一个 评价相位梯度成像方法新功能的II期临床研究。III期 商业开发将为临床医生提供增强的自适应光学成像能力， 在当前的视网膜成像仪中可用。

Project Summary/Abstract Powerful new Adapative Optics imaging technoIogies which sense ocular aberrations with a wave- front sensor and corrects them with a deformable mirror wave-front compensator, like Adaptive Optics Scanning Laser Ophthalmoscopy (AOSLO) and AO-OCT, provide detailed anatomical, physiological and functional information at the cellular level in the human eye. Physical Sciences Inc. (PSI) has recently demonstrated a new approach, called adaptive optics line scanning ophthalmoscopy (AO-LSO). The clinical AO-LSO retinal imager, invented and developed at PSI, greatly simplifies AO optical design, eliminates high-speed scanning components, and reduces the clinical footprint compared to research AOSLOs, while preserving many of the advantages of (line-)confocal imaging for resolving retinal layers. Although the AOSLO and AO-LSO provide the ability to routinely image and count cone photoreceptors, at present, there are no direct approaches for in vivo imaging of retinal ganglion cells in the human eye, or counting cell bodies in the inner retina. Our goal in Phase I is to demonstrate an advanced new design of the AO-LSO imager, (including an AO-OCT channel), with a new, high-sensitivity camera technology and line-illumination methods for phase gradient imaging of the inner retina. The envisioned platform will be engineered as a compact clinical system for flexible cellular imaging applications in vivo. An AO-LSO prototype instrument will be modified with new line-camera technology in Phase I, and used to investigate new imaging modalities. If successful, this approach and associated imaging algorithms will be improved in Phase II and used to monitor disease progression/regression and characterize inner retinal remodeling in patients at Children's Hospital Boston. A clinical investigator will provide feedback in Phase I and will lead a Phase II clinical study to evaluate the new capabilities of the phase gradient imaging approach. Phase III commercial development will provide clinicians with enhanced adaptive optics imaging capabilities not available in current retinal imagers.