Illumina HiSeq 2500 Sequencer

描述（由申请人提供）：当前申请申请购买Illumina HiSeq 2500测序仪的资金，这是下一代大规模并行测序平台。Illumina仪器的超高通量，加上大幅降低的测序成本，使其成为询问调节基因组序列和功能的首选平台。大规模平行测序特别适合于表观基因组学和功能基因组学研究，包括染色质结构、染色质可及性、组蛋白修饰和变异、调节因子定位；DNA甲基化;转录;基因组修饰的量化，如逆转录病毒载体整合的定位。HiSeq2500测序仪具有双流式细胞，在每个通道中生成高密度簇，每2X100 bp运行产生600 GB的高质量过滤数据。两个液流电池可以使用不同的化学试剂独立操作。该测序器具有在双模式下操作的能力；在快速运行模式下，在测序器上生成集群，允许产生多达单个或成对的150个碱基长读取，运行时间缩短了几倍，而在高输出模式下，每次运行的序列产量高达600 Gb。这允许根据速度和显著的大数据输出来调整序列运行以获得所需的结果。Illumina测序技术和化学技术的进步，每Mb序列输出的价格使HiSeq成为功能基因组学研究的首选平台，这些进步导致了大规模并行测序需求的爆炸式增长，因为更多的研究人员意识到该技术影响和加速他们研究的潜力。新工具将在一个完善的、自给自足的核心设施的范围内部署

DESCRIPTION (provided by applicant): The current proposal requests funds for the purchase of Illumina HiSeq 2500 sequencer, a next- generation, massively parallel sequencing platform. The ultra-high throughput of Illumina instruments, coupled with substantially reduced sequencing costs have made this a platform of choice for interrogating regulatory genomic sequences and function. Massively parallel sequencing is particularly well-suited for epigenomic and functional genomic studies, including chromatin structure, Chromatin accessibility, histone modifications and variants, regulatory factor localization; DNA methylation; transcription; and quantification of genome modifications such as localization of retroviral vector integrations. HiSeq2500 sequencer has dual flow cells with high density clusters generated in each channel that produces high quality passing filter data of 600 GB per 2X100 bp run. The two flow cells can be operated independently using different chemistries. The sequencer has the capability to operate in dual mode; in rapid run mode the clusters are generated on the sequencer, allows producing up to single or paired end 150 base long reads, and run times are several fold shorter , while in high output mode the sequence yields are up to 600 Gb per run. This allows tailoring the sequence run to the desired outcome in terms of speed vs. significantly large data output. The advances in Illumina sequencing technologies and chemistries, the price per Mb sequence output has made HiSeq a platform of choice for functional genomics research, These advances have led to explosive growth in demand for massively parallel sequencing, as more investigators realize the potential of the technology to impact and accelerate their research. The new instrument will be deployed in the context of a well-established, self-supporting core facility that already provides substantial epigenomics-focused next-generation sequencing services and associated bioinformatics support, and is therefore ideally positioned to rapidly translate the HiSeq 2500 potential to meet the needs of specific investigator projects as well as those of the general research community.