Adaptive Optics OCT/Fluorescence Small Animal Imager

描述（由申请人提供）：Physical Sciences Inc.提出开发一种新的集成多模式自适应光学小动物成像仪，包括AO-OCT和AO扫描激光荧光（fAOSLO），并展示其在检测啮齿动物眼病模型视网膜病变中的应用。该成像平台包括新颖的设计元素，例如可适应的波前传感方法，可在选定的层中实现动态和静态AO校正;集成Badal视力计系统，具有用于灵活AO-OCT引导成像的路径长度锁定OCT参考臂，以及用于增强麻醉啮齿动物的荧光灵敏度和共配准的共扫描AO-OCT/fAOSLO光栅。与波士顿儿童医院（CHB）的合作者一起，我们建议在STZ诱导的糖尿病视网膜病变的GFP小鼠（B6. 129 P-Cx 3cr 1 tm 1 Litt/J小鼠）模型中展示成像平台。快速增长的人类眼病的小动物模型阵列显著增加了对具有细胞分辨率的新的体内动物眼底成像模式的需求。然而，目前还没有商业化的AO平台用于可以利用这些特征的高分辨率体内成像。一个成功的I期项目将产生一个多模式自适应光学小动物成像（MAOSI）平台，同时具有AO-OCT、fAOSLO和改进的AO控制，用于3D结构和功能成像。这种装置具有直接的研究和药物发现应用和市场。

DESCRIPTION (provided by applicant): Physical Sciences Inc. proposes to develop a new, integrated multimodal adaptive optics small animal imager, including AO-OCT and AO scanning laser fluorescence (fAOSLO), and to demonstrate its applications to detecting pathology in the retinas of rodent models of eye disease. The imaging platform includes novel design elements such as adaptable wavefront sensing methods enabling dynamic and static AO correction in selected layers; integrated Badal optometer system with path-length-locked OCT reference arm for flexible AO-OCT-guided imaging, and co-scanning AO-OCT/fAOSLO rasters for enhanced fluorescence sensitivity and co-registration in anesthetized rodents. Together with collaborators at Children's Hospital Boston (CHB), we propose to demonstrate the imaging platform in a GFP mouse (B6.129P-Cx3cr1tm1Litt/J mice) model of STZ-induced diabetic retinopathy. The rapidly growing array of small animal models of human eye disease has significantly increased the need for new in vivo animal fundus imaging modalities with cellular resolution. However, there are currently no commercial AO platforms for high resolution in vivo imaging which can exploit such features. A successful Phase I program will result in a multimodal adaptive optics small animal imaging (MAOSI) platform with simultaneous AO- OCT, fAOSLO, and improved AO control for 3D structural and functional imaging. Such a device has immediate research and drug discovery applications and markets.