OMX Super Resolution Microscope

 描述（由申请人提供）：本申请是请求用于集成显微镜核心（IMC）的高速3D结构照明超分辨率荧光显微镜（SIM），IMC是贝勒医学院的长期共享仪器设施。所要求的OMX”系统与火焰”SIM-模块从应用精密是第一个商业上可用的超分辨率系统，能够获取3D图像的横向和轴向分辨率至少是两倍小于经典的限制所施加的衍射光，以与记录活细胞中的快速动态事件兼容的速度，并通过使用多个高分辨率-高灵敏度CMOS相机该系统允许3D成像的细节水平不可能与共焦显微镜或宽场3D去卷积系统。与生物学中常用的其他高分辨率成像工具（如电子和原子力显微镜、专用近场探针扫描显微镜以及其他新开发和商用的远场超分辨率技术（如STED、PALM和STORM））相比，OMX 3D SIM系统能够实现荧光成像，并且还针对图像采集速度和低曝光水平而构建与活细胞研究兼容。总的来说，OMX克服了其他显微镜系统的空间和时间限制。此外，OMX：a）与常规荧光染料一起工作，B）不存在对存在于其它高分辨率系统中的样品的几何限制，c）不需要特殊的安装和制备技术，以及d）操作起来不比已经在IMC中使用的其它市售显微镜系统复杂。OMX是市面上最快的3D SIM系统，也是唯一一款利用多个液冷sCMOS相机（最多4个）进行多波长成像的系统。OMX将被安置在IMC和邻近PI实验室的全新装修空间内。OMX除了IMC，在贝勒支持光学和电子显微镜的开放式光学成像设施，将协同和扩展当前最先进的成像和图像分析资源，包括硬件/软件培训和分析开发支持。

 DESCRIPTION (provided by applicant): The present application is to request a high speed multicolor 3D structured illumination super-resolution fluorescence microscope (SIM) for the Integrated Microscopy Core (IMC), a long standing shared instrumentation facility at the Baylor College of Medicine. The requested OMX" system with the Blaze" SIM- module from Applied Precision is the first commercially available super-resolution system capable of acquiring 3D images with lateral and axial resolutions at least two times smaller than the classical limit imposed by the diffraction of light, at speeds compatible with recording fast dynamic events in live cells and at multiple wavelengths by the use of multiple high resolution-high sensitivity CMOS cameras. The system allows 3D imaging at a level of detail not possible with confocal microscopes or wide field 3D deconvolution systems. In contrast to other high resolution imaging tools commonly used in biology, such as electron and atomic force microscopes, specialized near-field probe scanning microscopes and other newly developed and commercially available far-field super resolution techniques such as STED, PALM and STORM, the OMX 3D SIM system is able to achieve multicolor fluorescence imaging and is also built for image acquisition speeds and low levels of light exposure compatible with live cell studies. Collectively, the OMX overcomes spatial and temporal limitations presented by other microscopy systems. In addition, the OMX: a) works with conventional fluorochromes, b) does not present the geometrical limitations to the sample present in other high resolution systems, c) does not require special mounting and preparation techniques, and d) is not more complicated to operate than other commercially available microscopy systems already in use in the IMC. The OMX is the fastest 3D SIM system commercially available and is the only one that takes advantage of multiple liquid cooled sCMOS cameras (up to 4) for multi-wavelength imaging. The OMX will be housed in completely new renovated spaces for the IMC and adjacent PI's laboratory. An OMX addition to the IMC, an open access optical imaging facility at Baylor supporting light and electron microscopy, will synergize with and extend current state of the art imaging and image analysis resources, including hardware/software training and assay development support.