BIOTECHNOLOGY CORE

项目总结(见说明)： CCCR的生物技术核心将设在南卡罗来纳大学仪器资源设施(IRF)。IRF是一家成熟的生物技术核心，已有20年的历史，为研究人员提供了一系列最先进的设备，用于显微镜、细胞分选、小动物成像和几种分子技术。该机构有三名全职教员、两名兼职教员和四名技术人员，他们协助调查人员设计他们的实验和拨款提案，处理研究样本，操作设备，以及培训教职员工和学生。还提供了几个国家认可的讲习班和研究生级别的课程 通过国际扶轮基金会。IRF的资金来自各种来源，包括NIH用于购买主要设备的赠款、来自联邦和私人来源的各种资助项目的教职员工合作、用户费用和大学支持。生物技术核心的目标将通过三个具体目标实现：1)提供获得各种技术和主要设备的机会，包括但不限于流式细胞术和细胞分选、组织学、免疫组织化学、活细胞和共聚焦成像、电子显微镜以及小动物超声和荧光成像；2)提供技术专长，协助和培训CCCR研究人员及其实验室成员使用生物技术核心中现有的设备；以及3)通过小组会议和磋商，确定和准备获得新技术的提案，以使CCCR和其他研究人员的研究计划受益。

PROJECT SUMMARY (See instructions): The Biotechnology Core for the CCCR will be housed in the University of South Carolina Instrumentation Resource Facility (IRF). The IRF is an established biotechnology core, with a 20 year history, that provides a wide range of state-of-the-art equipment for microscopy, cell sorting, small animal imaging, and several molecular techniques for researchers. The facility is staffed with three full time faculty, two part time faculty, and four technicians that assist investigators with design of their experiments and grant proposals, processing of research samples, operation of equipment, and training of faculty, staff and students. Several nationally recognized workshops and graduate level courses are also available through the IRF. Funding of the IRF is generated from a variety of sources including grants from NIH for acquisition of major equipment, faculty collaborations on a variety of funding programs from federal and private sources, user fees, and University support. The goals of the Biotechnology Core will be addressed through three Specific Aims: 1) to provide access to a wide range of techniques and major equipment including, but not limited to, flow cytometry and cell sorting, histology, immunohistochemistry, live cell and confocal imaging, electron microscopy, and small animal ultrasound and fluorescence imaging; 2) to provide the technical expertise to assist and train CCCR Investigators and members of their laboratories on the use of equipment available in the Biotechnology Core; and 3) through group meetings and consultation identify and prepare proposals to acquire new technology which will benefit the research programs of the CCCR and other investigators.