FEI TITAN HALO 300kV INTERMEDIATE VOLTAGE ELECTRON MICROSCOPE

项目总结/摘要 该提案要求为国家显微镜和成像研究中心（NCMIR）提供资金， UCSD将获得300 keV FEI最先进的中高压电子显微镜（IVEM）， 将其与海量数据存储和计算资源集成。结果，有利于研究和 NIH生物医学研究人员的技术开发目标，将是一个集成的大数据应用程序， 通过广泛的三维（3D）体积进行体积可视化（纳米分辨率）。 这种耦合的显微镜/计算系统将被装备用于执行自动化的大体积3D 一系列生物医学研究项目所需的成像，包括分辨率下的全细胞成像 足以精确定位包含大分子复合物的分子构建单元，核内和 细胞质结构，并在其背景下表征生物材料的纳米结构， 功能环境。 该提案包括八个主要和三个次要的研究项目， 这台独一无二的新仪器可以完成的科学研究。 由于该仪器将生成大量的3D数据集， 基于计算的细化和定量分析，它将紧密耦合到一个大的，可扩展的数据， 存储和高性能计算环境，充分利用圣地亚哥 超级计算机中心和加州电信和信息技术研究所（两者都是 在UCSD）。 突出的国家，国际和本地用户将扩大这一平台的应用，以实现 最大限度地促进科学发现和人类健康。他们将共同努力，并与我们的 管理团队委托，使用和完善这台显微镜的功能。他们还将帮助 其他国家则充分受益于其能力。我们在设计和调试高端仪器方面的经验 我们的工作人员和科学家已经并将向制造商传达这些信息 目标是引导未来仪器的能力朝着对人类最有利的方向发展， 生物医学研究社区。

PROJECT SUMMARY/ABSTRACT This proposal requests funding for the National Center for Microscopy and Imaging Research (NCMIR) at UCSD to acquire a 300-keV FEI state-of-the-art intermediate-high voltage electron microscope (IVEM) and integrate it with massive data storage and computational resources. The result, to benefit the research and technology development aims of NIH biomedical investigators, will be an integrated big data appliance for volume visualization (to nanometer resolution) through extensive three-dimensional (3D) volumes. This coupled microscope / computational system will be equipped to perform automated large-volume 3D imaging required for a range of biomedical research projects, including imaging of whole cells at resolutions sufficient to pinpoint the molecular building blocks comprising macromolecular complexes, intra-nuclear and cytoplasmic structures, and characterization of nanostructures of biological materials in the contexts of their functional environments. This proposal includes eight major and three minor research projects that exemplify the transformative science that can be accomplished with this new one-of-a-kind instrument. Because this instrument will generate massive 3D datasets requiring substantial resources for computation-based refinement and quantitative analysis, it will be coupled tightly to a large, scalable data- storage and high-performance computing environment, leveraging the significant resources at the San Diego Supercomputer Center and the California Institute for Telecommunications and Information Technology (both at UCSD). Prominent national, international, and local users will broaden the application of this platform to achieve maximum impact in advancing scientific discovery and human health. They will work together and with our management team to commission, use, and refine the capabilities of this microscope. They will also help others benefit fully from its capabilities. Our experiences in designing and commissioning high-end instruments and those of our staff and scientists using them have been and will be communicated to the manufacturers with the goal of guiding the advance of the capabilities of future instruments in directions of most benefit to the biomedical research community.