Neural Cell Engineering & Imaging (NCEI) Core

摘要 神经细胞工程和成像核心（核心D，NCEI）一直是肯尼迪的重要组成部分， 中心成立25年来，资源和用户群稳步增长， 去年一年。NCEI核心由3个主要部门组成：细胞和组织工程子核心， 细胞/组织培养工作的设施和专业知识，包括原代、胚胎、iPS细胞和现代神经细胞 分化方案。现代显微镜SubCore，配备组织切片和成像仪器。 分析和图形子核心，它为工作站提供复杂的软件， 反卷积、定量2D到5D图像分析和重建、定制软件和宏，以及 作为图形和印刷服务。为了优化IDDRC调查人员对这些工具的使用，NCEI领导层 提供深入的用户咨询，强调精心策划的实验设计，并从事警惕 监测项目进展。核心的价值是通过与基因靶向专家顾问的联系而扩展的， 基因治疗、RNA构建体、光遗传学、电生理学和人ES、iPS和胎儿细胞。 通过与其他IDDRC核心的互动，协调，例如，研究 将脑细胞和解剖学变化与行为表型（AP）、遗传或表观遗传评估相关联 对分离的细胞亚群（NGEN）和对来自患者的重编程细胞（HCP）的翻译研究。 核心资源还通过提供成像和定量分析服务于22q11.2DS的U 54研究项目 分析基因表达的变化，分析小鼠模型中的神经亚型和树突的变化， 以及从携带22q11.2缺失的患者样品产生iPS细胞并分化神经元。的 核心通过定期的内部评估和年度用户评估来保持高标准的质量控制。 ADM Core进行的调查。总的来说，NCEI核心代表了科学的重要组成部分， 遗传学和认知表型之间的桥梁，因此对遗传学和认知表型具有广泛而重大的影响。 IDDRC研究人员进行的研究，重点是了解和治疗 IDDs.

ABSTRACT The Neural Cell Engineering and Imaging Core (Core D, NCEI) has been a vital component of the Kennedy Center for 25 years, steadily growing in resources and user base, with 110 individuals utilizing the Core in the last year alone. The NCEI Core consists of 3 major divisions: The Cell and Tissue Engineering SubCore, with facilities and expertise for cell/tissue culture work including primary, embryonic, iPS cells and modern neural differentiation protocols. The Modern Microscopy SubCore, with instruments for tissue sectioning and imaging. And the Analysis and Graphics SubCore, which provides workstations with sophisticated software for deconvolution, quantitative 2D to 5D image analyses and reconstruction, custom software and macros, as well as graphics and printing services. To optimize use of these tools for IDDRC investigators, NCEI leadership provides in-depth user consultation, emphasizes well-planned experimental design, and engages in vigilant monitoring of project progress. The Core's value is extended by ties with expert advisors on gene targeting, gene therapy, RNA constructs, optogenetics, electrophysiology, and human ES, iPS and fetal cells. Productivity and innovation is enhanced by interaction with other IDDRC Cores, coordinating, e.g., studies correlating brain cell and anatomic changes with behavioral phenotype (AP), genetic or epigenetic evaluations on isolated cell subpopulations (NGEN), and translational studies on reprogrammed cells from patients (HCP). Core resources also serve the U54 research project on 22q11.2DS by providing imaging and quantitative analyses of changes in gene expression, analysis of neural subtypes and dendritic changes in mouse models, and generating iPS cells and differentiating neurons from patient samples carrying 22q11.2 deletions. The Core maintains high standards of quality control through scheduled internal evaluations and the annual user survey carried out by the ADM Core. Collectively, the NCEI Core represents a significant part of the scientific bridge between genetics and cognitive phenotypes and as such has a broad and significant impact on the research carried out by IDDRC investigators that are focused on and critical to understanding and treating IDDs.