Mouse Genetics

摘要-小鼠遗传学共享资源 共享资源负责人：Kevin Kelley博士 Tisch癌症研究所（TCI）小鼠遗传学共享资源设施（MGSRF）为TCI成员提供 利用最先进的设施在小鼠中生产转基因和基因敲除癌症模型， 以及相关的啮齿动物胚胎学技术。MGSRF通过注射DNA产生转基因小鼠 分裂成原核阶段的单细胞小鼠胚胎，并协助实验室生产 通过使用小鼠胚胎干（ES）细胞或CRISPR/Cas9基因， 编辑.除了创造新的小鼠品系外，MGSRF还协助实验室维护 通过冷冻保存精子，以及从其他机构引进新的精子系， 通过从冷冻保存的胚胎或精子、活胚胎中回收小鼠，或通过使用IVF再衍生， 运送到ISMMS的雄性个体。MGSRF有着悠久的创造小鼠的历史， ISMMS的癌症相关研究及其在TCI计划中的作用将继续加强和提高 TCI成员使用新型突变小鼠模型进行尖端癌症研究的能力。

ABSTRACT – MOUSE GENETICS SHARED RESOURCE Shared Resource Leader: Kevin Kelley, PhD The Tisch Cancer Institute (TCI) Mouse Genetics Shared Resource Facility (MGSRF) provides TCI members with access to state-of-the-art facilities for the production of transgenic and knockout cancer models in mice, as well as related rodent embryology techniques. The MGSRF produces transgenic mice by injection of DNA fragments into pronuclear stage single-cell mouse embryos, and assists laboratories with the production of targeted mutant lines of mice through the use of mouse embryonic stem (ES) cells or CRISPR/Cas9 gene editing. In addition to the creation of novel mouse lines, the MGSRF assists laboratories with the maintenance of existing lines through the cryopreservation of sperm, and with the import of novel lines from other institutions by recovering mice from cryopreserved embryos or sperm, live embryos, or through the use of IVF rederivation of individual males that are shipped to ISMMS. The MGSRF has a long-standing history of creating mice for cancer-related research at ISMMS, and its role in the TCI programs will continue to strengthen and enhance the ability of TCI members to conduct cutting-edge cancer research using novel mutant mouse models.