Metabolism and Toxicity Core

项目总结(新陈代谢和毒性核心) 代谢和毒性核心的总体目的和功能是提供高质量的分析 支持路易斯维尔大学超级基金研究中心所有项目的服务和验证数据。 核心人员将对心血管生物标志物和指数进行测量和分析， 代谢和肝功能用于评估心脏代谢性疾病的风险和进展 接触挥发性有机化合物(VOCs)。核心人员还将提供智力投入和 就具体方法的选择提供咨询，并为学生、研究员、技术人员、 和初级教员。核心将使用严格的质量控制程序来验证数据和生成 统一数据报告和数据词典，储存在数据管理和分析核心。这个 CORE将通过其最先进的设备和分析为所有超级基金中心的研究项目提供支持。这个 核心提供的支持包括三个主要组件-用于检测的流式细胞仪组件 循环细胞和微粒的特性；用于测量生化的新陈代谢成分 和代谢终点，如尿VOCs代谢物，胆汁酸和儿茶酚胺的LC/MS/MS和 GC/MS；以及一种病理成分，使用一系列与血糖控制有关的测试来分析全身毒性， 肝功能和肝毒性。然而，核心项目的范围超出了项目的范围。 开发尖端技术和分析平台，以扩大其服务范围。这个 CORE将不断升级其基础设施和技术能力，以保持领先地位 与暴露和伤害的生物标记物有关的分析。核心也是致力于继续教育 它的用户以及大学的研究社区。该中心还将传播教育 关于其服务在促进环境健康方面的潜在效用和可获得性的信息 研究。通过确保统一和验证数据的集中存放，核心将提供坚实的基础 不仅是路易斯维尔大学超级基金调查人员的研究，还有其他超级基金的调查人员 中锋也是如此。因为它拥有最先进的设备，高度尖端的技术和熟练的 技术人员，核心将提供一流的毒理学和临床病理学服务。通过满足这些要求 为了实现目标，核心将继续成为中心内部增长和创新的重要工具，以及 教育中心用于培养环境健康研究领域的下一代科学家。

PROJECT SUMMARY (METABOLISM AND TOXICITY CORE) The overall purpose and the function of the Metabolism and Toxicity Core is to provide high quality analytical services and validated data in support of all projects at the University of Louisville Superfund Research Center. The Core personnel will conduct measurements and assays of biomarkers and indices of cardiovascular, metabolism, and hepatic function used for assessing the risk and progression of cardiometabolic disease upon exposure to volatile organic compounds (VOCs). The Core personnel will also provide intellectual input and advice in selection of specific methodological approaches and provide training to students, fellows, technicians, and junior faculty. The Core will employ strict quality control procedures to validate data and generate harmonized data reports and data dictionaries to be stored in the Data Management and Analysis Core. The core will support all Superfund Center research projects through its state-of-the-art equipment and assay. The support offered by the core encompasses three main components – a flow cytometry component for the detection and characterization of circulating cells and microparticles; a metabolism component for measuring biochemical and metabolic endpoints such as urinary metabolites of VOCs, bile acids and catecholamines by LC/MS/MS and GC/MS; and a pathology component to assay systemic toxicity using a battery of tests relating to glucose control, liver function and hepatic toxicity. However, the scope of the core exceeds that of the projects to encompass the development of cutting-edge techniques and analytical platforms to expand its repertoire of services. The Core will continually upgrade its infrastructure as well as its technical capabilities to remain at the leading edge of assays relating to biomarkers of exposure and harm. The core is also committed to the continued education of its users as well as the research community at the university. The Core will also disseminate educational information regarding the potential utility and accessibility of its services to enhance environmental health research. By ensuring central deposition of harmonized and validated data, the core will provide a firm foundation for research not only by the University of Louisville Superfund investigators, but investigators at other Superfund Centers as well. Because of its state-of-the-art equipment, highly sophisticated technologies, and skilled technical personnel, the Core will provide premier toxicological and clinical pathology services. By meeting these goals, the core will continue to be an essential vehicle for growth and innovation within the Center, as well as an educational hub for the training the next generation of scientists in the field of environmental health research.