Flow Cytometry Core

项目摘要 流式细胞术核心的目标是提供对最先进的流式细胞术(分类和 分析)为俄克拉荷马炭疽病小组的所有成员(U19)。流式细胞仪核心有两个高 快速荧光激活细胞分选仪(Beckman-Coulter MoFlo XDP和BD FACSAria)和三个 分析器(FACSCalibur、FACSCelesta和LSRII，均来自BD)。琳达·汤普森博士，她是 人体淋巴细胞的多色分析和分选，将担任核心主任和技术援助 由雅各布·巴斯先生和戴安娜·汉密尔顿博士提供。巴斯先生和汉密尔顿博士将执行所有类型的手术。 购买单抗的资金和使用费将提供给所有COBRE参与者。全 三个研究项目依赖于流式细胞术来实现他们的科学目标。因此，这个核心将是 大量使用，对俄克拉荷马炭疽集团的成功至关重要。

Project Summary The Aim of the Flow Cytometry Core is to provide access to state-of-the art flow cytometry (sorting and analysis) for all the members of the Oklahoma Anthrax Group (U19). The Flow Cytometry Core has two high speed fluorescence activated cell sorters (Beckman-Coulter MoFlo XDP and BD FACSAria) and three analyzers (FACSCalibur, FACSCelesta and LSRII, all from BD). Dr. Linda Thompson, who is an expert in multicolor analysis and sorting of human lymphocytes, will serve as Core Director and technical assistance will be provided by Mr. Jacob Bass and Dr. Diana Hamilton. Mr. Bass and Dr. Hamilton will perform all the sorts. Funds for the purchase of monoclonal antibodies and user fees will be provided to all COBRE participants. All three research projects depend upon flow cytometry to accomplish their scientific goals. Thus, this Core will be heavily used and is critical for the success of the Oklahoma Anthrax Group.