Center for Cellular Metabolism Research in Oklahoma

项目摘要/摘要 细胞代谢的改变导致了许多常见疾病的发病，这些疾病在 俄克拉荷马州。俄克拉荷马州细胞代谢研究中心(CMRO)第一阶段Cobre的目标是 在俄克拉荷马州创造和培育卓越的细胞新陈代谢。 两位现任CMRO Cobre研究项目负责人(RPL)(Lee博士和Cleuren博士)和我们的两位 即将毕业的RPL(于博士和赵博士)在他们的研究项目中使用共焦显微镜。此外，还有五个 CMRO Cobre的导师(夏博士、格里芬博士、奥尔森博士、斯里尼瓦桑博士和科瓦茨博士)经常使用共焦显微镜 用于他们的研究。2017年购买的尼康C2+共聚焦显微镜，用于支持8名调查人员 在OMRF的心血管生物学研究计划中，目前有13名研究人员使用，其中9人 与科布雷相关者(69.2%)。RPL和他们的导师是C2+的最大用户 并依靠这种设备免费为他们的研究制作一流的图像。 然而，C2+共焦开始显示出老化和过度使用的证据，其形式是 服务呼叫数和停机时间。此外，较新的共焦显微镜具有许多技术 C2+共焦中不可用的功能。例如，尼康AXR显微镜能够 以16倍更高的分辨率和2倍更高的信号灵敏度扫描更大面积的样品。这个 AXR还可以更快的速度获取图像，这是较大样本和实时成像的理想选择。 此外，AXR还能够对采集的图像进行去卷积，甚至可以提高分辨率 再远一点。本增刊要求部分支持新的尼康AXR共聚焦显微镜。OMRF将 提供设备总成本的50%。所要求的设备将减少对C2+的需求 ConFocus，支持我们的Cobre RPL建立具有竞争力的研究计划并过渡到 独立。

PROJECT SUMMARY/ABSTRACT Altered cellular metabolism contributes to the pathogenesis of many common diseases that are prevalent in Oklahoma. This Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE aims to create and nurture excellence in cellular metabolism in Oklahoma. Two of the current CMRO COBRE Research Project Leaders (RPLs) (Drs. Lee and Cleuren) and two of our graduating RPLs (Drs. Yu and Zhao) use confocal microscopy for their research programs. Additionally, five CMRO COBRE mentors (Drs. Xia, Griffin, Olson, Srinivasan, and Kovats) routinely use confocal microscope for their research. A Nikon C2+ confocal microscope that was purchased in 2017 to support eight investigators at the Cardiovascular Biology Research Program of OMRF is currently used by thirteen investigators, nine of whom are associated with COBRE (69.2%). The RPLs and their mentors are the heaviest users of the C2+ confocal and have relied on this equipment to produce top-notch images for their research at no cost. However, the C2+ confocal is starting to show evidence of aging and overuse in the form of increasing numbers of service calls and down time. Furthermore, the newer confocal microscopes have many technical capabilities that are not available in the C2+ confocal. For example, the Nikon AXR microscope is capable of scanning 2X larger area of the sample at 16X higher resolution and 2X higher sensitivity to the signal. The AXR can also acquire images at a much faster speed, which is ideal for larger samples and live imaging. Additionally, the AXR is capable of deconvoluting the acquired images and improving the resolution even further. This supplement requests partial support for a new Nikon AXR confocal microscope. OMRF will provide 50% of the total cost for the equipment. The requested equipment will reduce the demand on the C2+ confocal, supporting our COBRE RPLs in building their competitive research programs and transitioning to independence.