Comparative Biology of Tissue Repair, Regeneration and Aging

项目摘要 MDIBL的COBRE-II父母奖的目标是研究压力，衰老和 再生使用适当的模式生物系统的比较功能基因组研究。在 根据目前的建议，Murawala博士（MDIBL COBRE II的RPL）将与Arturo埃尔南德斯博士（教师）合作 科学家III，MHIR）和Joel Graber博士（MDIBL计算生物学核心主任）进行比较 研究了甲状腺激素诱导的美西螈和小鼠变态的影响， 方法论。Murawala博士将专注于了解甲状腺激素引起的蝾螈皮肤变化 利用10X Genomics的多组和空间转录组技术，埃尔南德斯博士将执行 在小鼠模型中进行补充实验，Graber博士将完善美西螈-小鼠的正向映射 并将使用这些更新的信息来分析和比较Murawala实验室的多组和空间数据 和埃尔南德斯实验室 这里提出的工作将使我们能够生成有价值的比较数据集，这些数据集将1）提供 对皮肤病理生理学的重要见解对异常甲状腺激素信号传导和2）形成的基础， 未来更大的赠款旨在阐明甲状腺激素调节的作用和潜在机制， 组织成熟和再生能力。

Project Summary The goal of the COBRE-II parent award at MDIBL is to study the intersection of stress, aging, and regeneration using comparative functional genomic studies of appropriate model organism systems. In the current proposal, Dr. Murawala (RPL within COBRE II at MDIBL) will team up with Dr. Arturo Hernandez (Faculty scientist III, MHIR) and Dr. Joel Graber (Director, MDIBL Computational Biology Core) to perform comparative studies the effect of thyroid hormone-induced metamorphosis in axolotl and mouse using next-generation methodologies. Dr. Murawala will focus on understanding the thyroid-hormone induced changes in axolotl skin using multiome and spatial transcriptome technology of 10X Genomics, Dr. Hernandez will perform complementary experiments in mouse models, and Dr. Graber will refine the axolotl-mouse orthology mapping and will use this updated information to analyze and compare multiome and spatial data from the Murawala lab and Hernandez labs. The work proposed here will allow us to generate valuable comparative datasets that will 1) provide crucial insights into pathophysiology of skin upon abnormal thyroid hormone signaling and 2) form the basis for future larger grants aimed at elucidating the role and underlying mechanisms by which thyroid hormones regulate tissue maturation and regenerative capacity.