Mouse Core

小鼠核心旨在为项目研究项目和核心提供动物育种，维护和表型资源，重点是WRN基因，基因组不稳定性和肿瘤发生。该核心以提高动物使用效率的方式解决了该计划项目的动物需求。两个共同的主题是普遍的：1)转基因小鼠的产生和使用，以及2)使用全动物试验来帮助表征肿瘤发展和其他相关表型的特征。Specific aim 1将产生新的胚胎干细胞和WRN错义突变的“敲入”小鼠模型，并确定它们的细胞和有机体表型。该中心将提供转基因技术、资源和专业知识，以产生新的小鼠品系。所有的资源都到位了

The Mouse Core is designed to provide animal breeding, maintenance, and phenotyping resources for program research projects and cores with a focus on the WRN gene, genomic instability and tumorigenesis. This core addresses the animal needs of the program project in a way as to increase the efficiency of animal use. Two common themes are prevalent: 1) the generation and use of genetically altered mice, and 2) the use of whole animal assays to help characterize features of tumor development and other related phenotypes. Specific aim 1 will generate new ES cell and 'knock-in' mouse models of WRN missense mutations and determine their cellular and organismal phenotypes. This core will provide the transgenic technology, resources and expertise to generate new lines of mice. All of the resources are in place to make targeting vectors, target mouse ES cells, generate chimeras and mutant mice, and genotype offspring to identify permanent founders. Specific aim 2 will maintain breeding colonies for new and established lines of mice. It is essential that the integrity of the genetic alteration of a specific line be maintained over several generations of back-crossing using sound breeding practices and accurate record keeping. In addition, basic observational assessments will be performed at the colony level to detect any unusual characteristics of newly generated mouse lines. Specific Aim 3 will conduct longitudinal studies on experimental cohorts of genetically defined mice. Phenotypic changes will be monitored and assessed in mice involved in project experiments. A systematic approach is necessary to detect phenotypic differences not easily discernible by casual observations. This core will provide mouse imaging resources as part of the phenotyping procedure. The need for an animal core is paramount in that all projects use mice. Consolidation of animal resources focused on maximizing efficiency and minimizing costs is essential to the success of this program project.