BINDING OF T4MO PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 福克斯实验室最近的研究表明，T4MOD(效应器蛋白)能够与T4MOC(Rieske铁氧还蛋白)结合，具有中等纳米摩尔亲和力。我们将利用这些蛋白质的现有结构来产生T4MOC和T4MOD的对接结构。15N HSQC滴定将确定每个蛋白质的相互作用面。黑线对接计划将被用来将两种蛋白质结构对接在一起。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent work in the Fox lab showed that T4MOD (the effector protein) is capable of binidng T4MOC (the rieske ferredoxin) with mid nano-molar affinity. We will leverage the existing structures of these proteins to generate docked structures of T4MOC and T4MOD. 15N HSQC titrations will determine the interaction face on each of the proteins. The program HADDOCK will be used to dock the two protein structures together.