Cytogenetics

细胞遗传学核心设施提供细胞遗传学、分子细胞遗传学和分子克隆 给希望之城癌症中心(CC)调查人员的化验。该设施的主要服务包括：细胞系 完整性、组织(细胞)培养、实体瘤细胞遗传学的表征和验证 基因组不稳定性、荧光显微镜和荧光原位杂交(FISH)研究 非整倍体检测、基因跟踪、特定基因改变、基因/表型靶向FISH(T-FISH) 用于残留病检测和基因整合/图谱研究的分析。光谱核型或24- 彩色核型、小鼠模型系统的20色核型和FICY(荧光 免疫表型和间期细胞遗传学作为研究肿瘤的工具)或T-FISH (连续表型，然后进行基因分析)也是可用的。酪胺信号放大 当怀疑目标水平较低时，FISH用于核酸的细胞定位；例如，在 基因或过继免疫疗法研究。由于CC会员的需要，我们将我们的服务扩展到 提供(1)克隆性检测以监测克隆性造血，克隆性造血是治疗相关急性白血病的先兆 部分接受剂量强化方案的患者出现髓系白血病/骨髓异常增生症； (2)用于综合分子核型分析的细菌人工克隆(BAG)阵列(ACGH)。我们也 为希望筛选自己的研究或需要的科学家提供成像分析培训 用途广泛。最后，这个核心也是SWOG细胞遗传学的参考FISH核心实验室 程序。在最近12个月报告所述期间，使用了细胞遗传学共享资源 来自所有5个研究项目的18名癌症中心成员。同行评审的使用率占97% 总使用量。这一核心的年度预算为292,811美元，其中22%是机构资金，22%是用户 手续费。26%来自其他来源，29%来自CCSG。

The Cytogenetics Core Facility provides cytogenetics, molecular cytogenetics, and molecular clonality assays to City of Hope Cancer Center (CC) investigators. Primary services of this facility include: cell line characterization and verification of integrity, tissue (cell) culturtng, solid tumor cytogenetics, assays for genomic instability, fluorescence photomicroscopy, and fluorescence in situ hybridization (FISH) studies for aneuploidy detection, gene tracking, specific gene alterations, genotype/phenotype targeted FISH (T-FISH) analyses for residual disease testing, and gene integration/mapping studies. Spectral karyotyping or 24- color karyotyping, 20-color karyotyping for mouse model systems, and FICTION (fluorescence immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasms) or T-FISH (sequential phenotypic followed by genotype analysis) are also available. Tyramide signal amplification FISH is used for the cellular localization of nucleic acids when target levels are suspected to be low; e.g., in gene or adoptive immunotherapy studies. Due to CC members' needs, we expanded our services to provide (1) clonality testing to monitor for clonal hematopoiesis, a precursor of therapy-related acute myelogenous leukemia/myelodysplasia observed in some patjents who received dose-intensive regimens; and (2) Bacterial Artificial Clones (BAG) arrays (aCGH) for comprehensive molecular karyotyping. We also provide imaging analysis training for scientists who wish to screen their own studies or who require extensive usage. Finally, this core is also the reference FISH core laboratory for the SWOG cytogenetics program. During the most recent 12-month reporting period, the Cytogenetics shared resource was used by 18 Cancer Center members from all 5 Research Programs. Peer-reviewed usage represented 97% of total usage. Annual budget for this core is $292,811, of which 22% is institutional funding, 22% is user fees. 26% is from other sources, and 29% is requested from the CCSG.