BOOSTING HBV RESPONSES IN BABOONS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在狒狒中进行的这项研究旨在测试引发免疫对后续加强免疫应答的影响。 在本研究选择的6只动物中，3只先前已用市售疫苗免疫，3只已用供试品免疫。 市售疫苗和供试品均为含有B型肝炎表面抗原（HBsAg）的B型肝炎疫苗。 我们想看看当用低剂量的B型肝炎表面抗原加强一次时，反应是否有差异。 疫苗制剂将通过四头肌内注射给药。 在免疫后第2周和第6周采集血样，以测定这些时间点血清中的抗HBsAg抗体滴度。 因为这些动物已经用HBsAg致敏，我们预期低剂量免疫将诱导强烈的免疫应答。 该项目将使医学受益，因为它将表明与标准商业疫苗相比，测试疫苗建立的记忆免疫反应有多强。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study in baboons is designed to test the effect of priming immunization on subsequent boost responses. Of the six animals selected for this study, three have previously been immunized with the commercial vaccine, and three have been immunized with the test article. Both the commercial vaccine and the test article are hepatitis B vaccines containing the hepatitis B surface antigen (HBsAg). We would like to see if there is a difference in response when boosted once with a low dose of hepatitis B surface antigen. The vaccine formulation will be administered by intramuscular injection in the quadriceps. Bleeds will be collected at both week 2 and week 6 post immunization to determine the anti-HBsAg antibody titers in the sera at these time points. Because these animals have already been primed with HBsAg, we expect that immunization with a low dose will induce a strong immune response. The project will benefit medicine as it will provide an indication of how potent the memory immune response set up by the test vaccine is compared to the standard commercial vaccine.