CLINICAL TRIAL: SERUM PROTEOMIC PATTERNS IN NECROTIZING ENTEROCOLITIES IN PRETER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 坏死性小肠结肠炎(NEC)是一种与早产儿肠壁缺血和坏死相关的疾病。它是影响早产儿的最常见的长期疾病之一。1000g婴儿中NEC的发生率为7%。疾病的病因是多因素的；肠壁缺血、肠道细菌定植和进食通常在疾病发生之前就出现了。目前我们对NEC分子发病机制的认识有限。 本研究的目的是利用蛋白质组学和生物信息学技术：1)识别易患NEC的血清低分子量蛋白质生物标志物；2)识别参与NEC炎症级联反应的低分子蛋白质。 将从早产母亲身上采集血液，并在开始喂养前、出现NEC样症状时和NEC发病后24小时从出生3天和7天的早产儿身上采集脐带血。样品将储存在-70C下，并同时使用表面增强激光解吸电离飞行时间质谱仪(SELDI-TOF-MS)进行分析。将对样本中存在的数千种蛋白质中的一小部分蛋白质或多肽形成的识别模式进行分析，以建立最有可能与NEC有关的特征模式。这项研究的结果将应用于掩蔽样本，作为更大规模多中心试验的一部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Necrotizing enterocolitis (NEC) is a condition that is associated with ischemia and necrosis of the bowel wall in preterm infants. It is one of the most common long-term morbidities affecting premature infants. The incidence of NEC is 7% in < 1000 g infants. The etiology of the disease is multifactorial; bowel wall ischemia, bacterial colonization of the intestine and presence of feedings are usually present prior to the onset of the condition. Our current understanding of the molecular pathogenesis of NEC is limited. The goal of this study is use proteomics and bioinformatic technologies to: 1) identify serum low molecular weight protein biomarkers that predispose an infant to NEC, and 2) to identify low molecular weight proteins that participate in the inflammatory cascade in NEC. Blood will be collected from mothers in preterm labor and cord blood from preterm infants at 3 and 7 days-of-age, prior to initiation of feeds, at onset of NEC-like symptoms and 24 hrs after onset of NEC. Samples will be stored at -70C and analyzed simultaneously using surface enhanced, laser desorption ionization time of flight mass spectroscopy (SELDI-TOF-MS). The discriminating pattern formed by a small subset of proteins or peptides among the thousands of proteins present in the sample will be analyzed to establish a signature pattern that is most likely to be associated with NEC. Results from this study will be applied to masked samples as a part of a larger multicenter trial.