AUTOMATED FINDING OF MODIFIED PEPTIDES FROM TANDEM MS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 利用PTM或氨基酸突变对多肽进行修饰对许多领域的研究具有重要意义。信号转导中的磷酸化和蛋白质损伤中的氧化都是例子。这些修饰会影响蛋白质的结构和活性，如果人工诱导，可以用来研究蛋白质的结构。在许多情况下，修饰的确切性质是未知的，或者有太多的修饰而不考虑使用蛋白质数据库搜索程序。编写了一个通用程序，用于发现任何类型的修改。它基于产物离子光谱与理论光谱或现有未修饰多肽光谱的2D相关性。使用用于相关性分析和定位修改位置的查看器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Peptide modification by PTM or by mutation of amino acids is important to researcher in many fields. Phosphorilation in signaling and oxidation in protein damage are examples. These modifications affect protein structure and reactivity and can be used to study protein structures if induced artificially. In many cases the exact nature of the modification is not known or there are too many of them to consider using a protein database search program. A general program for discovering any kind of modification was written. It is based on 2D correlation of product-ion spectra to theoretical spectra or to existing spectra of unmodified peptides. A viewer for correlation analysis and locating the modification position is used.