CORE--PROTEOMICS FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 西特曼癌症中心(SCC)蛋白质组学核心使用当前和新开发的方法和试剂为SCC成员提供多路蛋白质组分析。这些方法包括用多种荧光染色定量和检测官能团的二维差示凝胶电泳法，用串联质谱仪(LC-MS/MS和MALDI-TOF/-TOF)鉴定蛋白质的方法，以及用差示稳定同位素标记的多维液相色谱方法。SCC蛋白质组学核心正在与SCC生物信息学核心(核心总监Jeffrey Milbrandt)和多重基因分析核心(核心总监Mark Watson)合作，建立一个实验室信息管理系统(LIMS)和一个关系数据库，使SCC研究人员能够通过单一的医疗信息门户访问和查询临床、蛋白质组学和mRNA阵列数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.