DECIDUAL MACROPHAGES IN PRIMATE PREGNANCY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：检验如下假设：蜕膜巨噬细胞与绒毛外滋养层细胞的相互作用可通过表达于恒河猴滋养层细胞上的非经典MHC I类分子Mamu-AG与表达于巨噬细胞上的白细胞免疫球蛋白样受体的相互作用来介导。 白细胞免疫球蛋白样受体（LILR）是一类具有抑制和激活功能的受体家族，广泛表达于淋巴细胞和骨髓细胞。为了鉴定恒河猴LILR，我们进行了恒河猴脾脏和蜕膜cDNA文库的筛选和RT-PCR克隆。我们获得了8个不同的全长克隆，其结构和功能多样性类似于人LILR，包括具有基于免疫受体酪氨酸的抑制基序的LILR，具有在跨膜结构域中含有带正电荷的精氨酸残基的截短的胞质尾部的LILR，以及缺乏跨膜或胞质结构域的推定的可溶性受体。恒河猴、黑猩猩和人类LILR基因的系统发育树分析揭示了Mm-LILRAd和hLILRA 3以及Mm-LILRBc和hLILRB 4之间的两个不同的簇和直系关系。恒河猴LILR的表征将有助于使用这种非人灵长类动物模型研究LILR的功能作用，包括通过与非经典MHC I类分子相互作用的免疫调节。 这项研究使用WNPRC研究服务。 NIH/NICHD对这个五年期项目的资助截止于2008年3月，就在本报告所述期间开始之前。出版物待定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To test hypothesis that the interaction of decidual macrophages with extravillous trophoblasts can be mediated through interaction of the non-classical MHC class I molecule Mamu-AG, expressed on rhesus trophoblasts, with leukocyte immunoglobulin-like receptors, expressed on macrophages. Leukocyte Ig-like receptors (LILRs) are a family of receptors that have inhibitory and activating functions and widely expressed by lymphoid and myeloid cells. To identify the rhesus monkey LILRs we performed screening of rhesus spleen and decidua cDNA libraries and RT-PCR cloning. We obtained eight different full length clones with structural and functional diversity similar to human LILRs, including LILRs with immunoreceptor tyrosine-based inhibitory motifs, LILRs with truncated cytoplasmic tails containing positively charged arginine residues in the transmembrane domain, and putative soluble receptors lacking transmembrane or cytoplasmic domains. Phylogenetic tree analysis of rhesus, chimpanzee and human LILR genes reveals two distinct clusters and orthologous relationship between Mm-LILRAd and hLILRA3, and Mm-LILRBc and hLILRB4. Characterization of rhesus LILRs will facilitate use of this nonhuman primate model for the study of the functional role (s) of LILRs, including immune regulation through interaction with non-classical MHC class I molecules. This research used WNPRC Research Services. NIH/NICHD funding for this five-year project ended March 2008, just before this reporting period began. Publications are pending.