Biological

项目总结（见说明）： 生物成像设备（BIF）为用户提供设备和技术专长，以进行广泛的成像实验，从超微结构水平（电子显微镜（EM）），细胞（光学显微镜（LM））到动物组织和器官（小动物成像（SAI））。BIF是通过合并先前单独批准的CCSG细胞成像设施、电子 显微镜（EM）和小动物成像服务。此次合并是基于自上次审查以来创建的设施母公司监督委员会（FPOC）的建议，以帮助优先考虑共享资源。FPOC指出，建立BIF将合并以前三个设施的重叠科学目标，从而通过精简行政和技术来降低运营成本。 在不牺牲原有设施所提供的任何服务的情况下，该设施提供的服务可应用于临床、转化和基础研究。这反映在整个中心对基金资源的使用上。自上次审查以来，BIF被来自该中心所有五个研究项目的39名用户使用，其中37名是同行评审资助的研究人员。这个设施 提供了必要的技术和科学支持，因为它产生的数据出现在60个同行评审的出版物中，其中18个报告了部分由BIF促进的互动促进的合作研究。 该基金是中心的一项非常宝贵的资源，这一点从机构资金对资本设备的重大投资中可以看出。在此资助期间购买的设备包括多光谱共聚焦显微镜，光谱核型（SKY）设备，用于解剖成像的高场MRI系统（7特斯拉布鲁克DRX），检测荧光素酶或荧光标记细胞的Caliperls IVIS光谱， 和Visen FMT 2500，其检测活动物中酶促活化的光学探针（金属蛋白酶、组织蛋白酶、弹性蛋白酶）或荧光标记探针的三维分布。 这些新技术使用户能够在组织、细胞和亚细胞各级收集实时信息，从而大大扩展了该设施的能力。设备投资沿着由专职人员进行的测定和方案开发，继续为用户提供最新的 成像技术的进步。因此，这些重要的服务加强了研究人员的研究计划，并促进了内部和跨计划的互动。

PROJECT SUMMARY (See instructions): The Biological Imaging Facility (BIF) provides users with the equipment and technical expertise to conduct a wide array of imaging experiments that span from the ultrastructural level (Electron Microscope (EM)), cellular (Light Microscope (LM)) to tissues and organs in animals (Small Animal Imaging (SAI)). The BIF was created by merging previously separate approved CCSG Facilities for Cell Imaging, Electron Microscope (EM) and Small Animal Imaging services. The merger was based upon the recommendation of the Facility Parent Oversight Committee (FPOC) that was created since the last review to help prioritize shared resources. The FPOC noted that creating the BIF would consolidate overlapping scientific objectives of the three previous facilities and thus reduce operating costs by streamlining administrative and technical operations, without sacrificing any of the services that were offered by the original facilities. The services offered by this Facility have applications in clinical, translational and basic research. This is reflected by the center-wide use of the Facility resources. Since the last review, the BIF was utilized by 39 users from all five of the Center's Research Programs, 37 of whom were peer-review funded investigators. This Facility provides essential technical and scientific support as it has generated data that appeared in 60 peer reviewed publications, 18 of which reported collaborative studies that were fostered in part by interactions facilitated by the BIF. This Facility is a highly valued resource at the Center as is evident by the significant investment of Institutional funds for capital equipment. Equipment purchased during this funding period include a multispectral confocal microscope, Spectral Karyotype (SKY) equipment, a high-field MRI system (7-Tesla Bruker DRX) for anatomic imaging, a Caliperls IVIS spectrum that detects luciferase or fluorescently tagged cells in animals, and a Visen FMT2500 which detects the three dimensional distribution of enzymatically activated optical probes (metalloproteinases, cathepsin, elastase) or fluorescently tagged probes in live animals. These new technologies significantly expanded the capabilities of this Facility by providing users the ability to collect real-time information at the tissue, cellular and subcellular levels. The investment in equipment along with assay and protocol development conducted by a dedicated staff continue to provide users with the latest advances in imaging technologies. These vital services thus enhance the research programs of investigators and facilitate intra and interprogrammatic interactions.