Husbandry and Management Core-Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

项目总结/摘要 畜牧业和管理核心 耶基斯国家灵长类动物研究中心（YNPRC）拥有悠久而成功的育种历史 印度恒河猴用于艾滋病研究。这一成功部分归功于持续的 U24/U42支持管理和照顾这个殖民地。自2002年以来，这种支持使恒河猴 猕猴繁殖群体在耶基斯NPRC野外站建立一个群体谱系，过渡到全面 SPF状态，以建立MHC谱，并验证快速内部疱疹B病毒（猕猴疱疹病毒 1)筛选方案。在过去4年中，国家卫生研究院资助的艾滋病毒/艾滋病相关项目的数量 需要SPF恒河猴的数量保持相对稳定，我们现在看到COVID后的增加 动物的请求。因此，当前U42的畜牧业和管理核心的主要目标是 应用是利用U42提供的资源，最大限度地提高SPF印度恒河猴的产量 猴子，并促进分配这些动物用于NIH资助的艾滋病毒/艾滋病研究。作为目标1的一部分， 畜牧和管理核心将继续保持SPF状态和遗传健康， 群体殖民地的身体和社会健康，以生产具有所需MHC基因型的高质量动物 用于艾滋病研究。作为目标2的一部分，最大限度地提高动物生产潜力， 管理核心将修订战略，以提高年轻的未来男性育种者的社会能力 和形成多个雄性群体的育种，以提高男性引进的成功率，并实施一个 社会网络分析方法，以评估我们的大型，化合物圈养繁殖群体的稳定性，如 打破母系制度，以改善社会稳定，避免社会不稳定带来的不利影响。 在生产上。最后，作为目标3的一部分，采取综合方法管理菌落数据集， 畜牧业和管理核心将从存储社会化和社会行为数据集转变为 在分隔的电子表格，而不是存储在网络访问的殖民地信息资源 并实施项目管理仪表板，以便于操纵和汇总有关 动物可用性、请求和分配，以便于跟踪和报告。这些目标的成功 将确保YNPRC的SPF菌落将继续增长，并提供可靠的健康和 遗传特征良好的恒河猴用于艾滋病毒/艾滋病研究。

Project Summary/Abstract Husbandry and Management Core The Yerkes National Primate Research Center (YNPRC) has a long and successful history of breeding rhesus macaques of Indian origin for HIV/AIDS research. This success can be attributed, in part, to continuous U24/U42 support for the management and care of this colony. Since 2002, this support has allowed the rhesus macaque breeding colony at the Yerkes NPRC Field Station to establish a colony pedigree, to transition to full SPF status, to institute MHC profiling, and to validate a rapid in-house Herpes B virus (Macacine herpesvirus 1) screening protocol. Over the past 4 years, the number of individual NIH-funded HIV/AIDS-related projects requiring SPF rhesus monkeys has remained relatively steady, and we are now seeing a post-COVID increase in animal requests. Thus, the main objective of the Husbandry and Management Core for the current U42 application is to leverage the resources provided by the U42 to maximize production of SPF Indian rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. As part of Aim 1, the Husbandry and Management Core will continue to maintain the SPF status and genetic health as well as the physical and social health of the Colony in order to produce high quality animals with desired MHC genotypes for HIV/AIDS research. As part of Aim 2 to maximize animal production potential, the Husbandry and Management Core will revise the strategies for improving the social competence of young future male breeders and for forming multi-male cohorts of breeders to improve the success of male introductions, and implement a social network analytical approach to assess stability in our large, compound-housed breeding groups, such as fragmentation in matrilines, to improve social stability and avoid the detrimental effect that social instability has on production. Finally, as part of Aim 3 to take an integrated approach to managing colony data sets, the Husbandry and Management Core will transition away from storing socialization and social behavioral datasets in compartmentalized spreadsheets to instead store them in the web-accessible Colony Informatics resource and implement a project management dashboard to facilitate manipulation and summary of data regarding animal availability, requests, and assignments to facilitate tracking and reporting. The success of these aims will ensure that the SPF colony at YNPRC will continue to grow and provide a reliable supply of healthy and genetically well-characterized rhesus macaques for HIV/AIDS research.