Core B: Liver Cell Isolation Core

项目总结/摘要 肝细胞分离中心，前身为人类细胞分离中心，是美国唯一一个由COBRE资助的设施。 美国致力于从人类、小鼠和大鼠中分离肝细胞。肝细胞分离核心致力于 实现以下目标。1)提供分离的肝细胞和特异性非实质肝细胞， 正常和患病的人类和啮齿动物肝脏，用于支持科学研究、赠款和出版物。（二） 通过与堪萨斯大学肝脏中心的合作关系，成为一个更全面的核心。 3)建立一个核心实验室，通过生成自己的 收入和通过其他来源的机构和/或NIH的发现。核心在一种状态下运作， 位于堪萨斯大学医学中心校园内的艺术实验室。核心提供专家建议 关于肝细胞和非实质细胞的分离、培养和维持。核心是 在Hartmut Jaeschke博士的指导下，由核心技术总监Kenneth Dorko先生担任工作人员， 以及一名核心技术员蔡慧娜女士。对于COBRE第三阶段，肝细胞分离核心将扩大 获得肝脏标本的机构数量，增加使用其 资源，并逐步提高其服务费。这些行动，加上其他机构和 未来NIH的支持将使核心能够自我维持。

PROJECT SUMMARY / ABSTRACT The Liver Cell Isolation Core, formerly the Human Cell Isolation Core, is the only COBRE funded facility in the U.S. dedicated to the isolation of liver cells from humans, mice and rats. The Liver Cell Isolation Core strives to meet the following goals. 1) To provide isolated hepatocytes and specific non-parenchymal liver cells from normal and diseased human and rodent livers for the support of scientific studies, grants and publications. 2) To become a more comprehensive core by a collaborative affiliation with the University of Kansas Liver Center. 3) To establish a core lab that can operate without COBRE grant support through generation of its own revenue and through other sources of institutional and/or NIH finding. The Core operates out of a state of the art lab located on the campus of the University of Kansas Medical Center. The Core provides expert advice regarding the isolation, culture and maintenance of hepatocytes and non-parenchymal cells. The Core is under the direction of Dr. Hartmut Jaeschke and is staffed by a Core Technical Director, Mr. Kenneth Dorko, and a Core Technician, Ms. Huina Cai. For COBRE Phase III, the Liver Cell Isolation Core will expand the number of institutions from which it obtains liver specimens, increase the number of investigators that use its resources and gradually increase its service fees. These actions, coupled with additional institutional and future NIH support will allow the Core to become self-sustaining.