Repurposing Bacterial Mechanosensitive Channel as a Membrane Tension Biosensor - Diversity Supplement

项目摘要 我们正在请求一份研究补充资料，以促进与健康相关的研究的多样性，以 补充母体R21授权(1个R21 GM134157-01)，标题为 作为一种膜张力生物传感器的机械敏感通道。补充资金为 建议支持应用专业二年级博士生Grace Cai 密歇根大学物理学专业。格蕾丝有潜力为 父母基金的首要目标是寻求开发一种膜张力生物传感器 基于细菌机械敏感基因工程的机械敏感细胞功能研究 MSCL频道。格雷斯热衷于通过以下方式为大学的多样性做出贡献 她参加的各种外展/志愿者活动。作为一名第一代大学生 作为一名正在攻读物理课程的女学生，格蕾丝将成为 其他对物理和/或生物物理学感兴趣的女学生。她的观点将是 在学术环境中有价值，并将丰富我们的社区。我们已经制定了一项计划 在这段支持期间，她作为一名科学家一起进行研究和职业发展，并 更远一点。由于格蕾丝来自物理背景，没有在 生物学之前，我们已经进行了几项培训活动，旨在扩大她的 技术知识和技能，共同帮助格雷斯成为一个独立的 研究员。格雷斯将有机会在地方会议和全国会议上发表演讲。 会议。GRACE将产生MSCL构建，将它们转导到不同的细胞系中，以及 评价工程化膜张力生物传感器的表达和功能。她有 最近学到了一种新的增加膜张力的微操作装置，并获得了 细胞生物学技能，并将两者付诸实践。格蕾丝的贡献将推动这一目标 在1个R21 GM134157-01资助中。

Project Summary We are requesting a Research Supplement to Promote Diversity in Health-Related Research to complement the parent R21 grant (1 R21 GM134157-01) entitled ‘Repurposing Bacterial Mechanosensitive Channel as a Membrane Tension Biosensor’. The supplement funds are proposed to support Grace Cai, who is a second year Ph.D. student in the Program of Applied Physics at the University of Michigan. Grace has the potential to contribute significantly to the overarching goal of the parent grant that seeks to develop a membrane tension biosensor for studying mechanosensitive cell functions based on engineering a bacterial mechanosensitive channel MscL. Grace is enthusiastic about contributing to the diversity of the University through the various outreach/volunteer activities she participates in. As a first-generation college student and a female student now enrolled in a physics program, Grace will serve as a role model for other female students interested in pursuing physics and/or biophysics. Her perspective will be valuable in an academic environment and will enrich our community. We have developed a plan together for her research and career development as a scientist during this support period and beyond. Since Grace comes from a physics background without having done research in biology before, we have put in place several training activities that aim to broaden both her technical knowledge and skills that together will help Grace becomes an independent researcher. Grace will have an opportunity to present at a local meeting as well as at a national conference. Grace will generate MscL constructs, transduce them into different cell lines, and evaluate the expression and function of the engineered membrane tension biosensors. She has recently learned a new micromanipulation device for increasing membrane tension and acquired cell biology skills and will put both into practice. Grace’s contributions will advance the objective of the 1 R21 GM134157-01 grant.