Phosphorelay signaling and regulation in bacteria

大肠杆菌和相关细菌使用多步磷光继电器来感测细胞内和细胞外 环境信号和调节不同的细胞过程。然而，多重的意义在于 表征这些体系的磷酰基转移步骤仍然知之甚少。作为我们长期- 学期目标是了解细菌使用的各种信号转导系统之间的相互作用 为了适应不同的环境条件，本提案将探索这些系统中的几个 特别容易处理，这是由于建立了良好的投入和/或产出，并在 大肠杆菌生理学。主要的焦点将放在两个对过渡到 厌氧，Arc和Tor，以及调节与氮相关的磷酸组氨酸磷酸酶 磷酸转移酶系统。了解这些网络的进展将为我们对这两种网络提供新的见解 病原体感染和维持宿主内健康微生物区系的机制 利基市场。这一结果可能最终导致新的抗生素或治疗方案的开发，如 以及操纵微生物群以维持宿主健康的策略。

Escherichia coli and related bacteria employ multi-step phosphorelays to sense intra- and extra-cellular environmental signals and to modulate diverse cellular processes. However, the significance of the multiple phosphoryl transfer steps that characterize these systems remains poorly understood. As part of our long- term goal to understand the interplay between the various signal transduction systems that bacteria employ to adapt to diverse environmental conditions, this proposal will explore several of these systems that are particularly tractable, due to well-established inputs and/or outputs, and that play critically important roles in E. coli physiology. The primary focus will be on two phosphorelays that are important for the transition to anaerobiosis, Arc and Tor, as well as a phosphohistidine phosphatase that modulates the nitrogen-related phosphotransferase system. Progress in understanding these networks will provide new insights into both the mechanisms enabling infections by pathogens and the maintenance of a healthy microbiota in host niches. The results may ultimately lead to the development of novel antibiotics or treatment regimens, as well as strategies for manipulating the microbiomes to maintain the health of the host.