The Stanford-SLAC CryoEM Center

项目摘要 斯坦福大学和SLAC国家加速器实验室(SLAC)提议主办一场 国家低温电子显微镜中心-斯坦福-SLAC低温电子显微镜中心(S2C2) -满足国家对低温EM作为原子分辨结构工具的新需求 生物学。拟议的S2C2将(1)建立并保持最先进的低温- 满足用户对原子分辨率图像数据的需求的电子显微镜，(2)档案图像 具有适当元数据的数据，(3)提供计算资源以实时评估数据质量 时间，(4)告知和招募美国各地的潜在用户关于公共可访问性的信息 中心，(5)建立公开、公平、透明和高效的程序来选择用户提案 基于科学影响和样本准备情况，无论用户的地理位置或 (6)帮助用户克服技术障碍，使他们能够获得更高的 使用严格的验证协议快速解析CryoEM结构，(7)形成并促进 用户网络无缝交换信息，(8)培养用户独立 CryoEM调查员，(9)将用户和受训人员的反馈整合到一个连续的设施循环中 加强，以及(10)优化操作，以有效地完成上述任务和 效率很高。华秋博士，联系人派，拥有数十年的高分辨率开发经验 冷冻EM技术，并指导多个机构的研究和培训企业。 迈克尔·施密德博士和布里特·海德曼博士也是该中心的私人助理，他们带来了互补的专业知识 在低温电子显微镜、结构生物学和国家大型用户的管理和运营中 设施。一名中心运营总监，三名熟练的低温EM专家，一名技术助理， 而一名用户设施管理员将对S2C2的S进行日常操作，包括数据 收集、质量评估和交叉培训。此S2C2将驻留在位于 SLAC。它将利用SLAC的专业知识，每年在其著名的 X射线同步加速器和自由电子激光用户设施。由以下成员组成的咨询委员会 外部专家科学家以及用户代表将提供有关S2C2的建议 运营和开发。我们的中心将建立在独一无二的优势和来自 斯坦福大学和SLAC，并将创造一个独特的环境，结合高吞吐量 通过培训下一代低温EM科学家来生成CryoEM数据。

Project Summary Stanford University and the SLAC National Accelerator Laboratory (SLAC) propose to host a National Center for Cryogenic electron Microscopy – The Stanford-SLAC CryoEM Center (S2C2) – to meet the emerging national need for cryoEM as a tool for atomic-resolution structural biology. The proposed S2C2 will (1) establish and keep at the forefront, state-of-the-art cryo- electron microscopes to satisfy users’ needs for atomic-resolution image data, (2) archive image data with appropriate metadata, (3) provide computing resources to assess data quality in real time, (4) inform and recruit potential users across the U.S. about the public accessibility of the Center, (5) establish an open, fair, transparent and efficient process to select user proposals based on scientific impact and specimen readiness, regardless of users’ geographic locations or affiliations, (6) help users to overcome technical hurdles and enable them to obtain high- resolution cryoEM structures quickly with a rigorous validation protocol, (7) form and facilitate a user network to exchange information seamlessly, (8) train users to become independent cryoEM investigators, (9) integrate user and trainee feedback into a continuous loop of facility enhancement, and (10) optimize operations to achieve the above tasks effectively and efficiently. Dr. Wah Chiu, the Contact PI, has decades of experience developing high-resolution cryoEM technologies and directing research and training enterprises across multiple institutions. Drs. Michael Schmid and Britt Hedman, also PIs of the Center, bring complementary expertise in cryoEM, structural biology, and management and operation of large-scale national user facilities. A Center Director of Operations, three skilled cryoEM specialists, a technical assistant, and a user facility administrator will carry out the S2C2’s day-to-day operations, including data collection, quality assessment, and cross-training. This S2C2 will reside in a new building at SLAC. It will leverage SLAC’s expertise in serving thousands of users each year at its renowned X-ray synchrotron and free electron laser user facilities. An Advisory Committee composed of external expert scientists as well as user representatives will provide advice regarding S2C2 operations and development. Our Center will build on the unique strengths and support from Stanford and SLAC, and will create a unique environment that combines high throughput cryoEM data generation with training the next generation of cryoEM scientists.