CSHL Single Cell Analysis Course (2023-2027)

摘要 CSHL单细胞分析实验室课程 拟议的冷泉港实验室(CSHL)课程单细胞分析每年举行一次 2023年7月至2027年7月，是CSHL细胞和分子生物学研究生培训计划的一部分。 这是一个为期两周的短期强化实践课程，为学生直接进入 利用当代先进和/或专门的分子技术进行的研究 单细胞研究的方方面面。本课程将涵盖的一般主题包括 RNAseq单细胞定量分析、基因组DNA分析、蛋白质组学和代谢组学。 本课程讲授的具体实验技术包括：1)基于液滴和微孔的单孔 细胞分离；2)单细胞RNA序列文库制备；3)单分子FISH；4)单细胞 多肽；5)单细胞蛋白质印迹；6)光活化单细胞探针；7)单细胞团 光谱分析；8)软X射线断层扫描；9)介绍性单细胞测序分析。实验 技术是在它们可能提供的更广泛的概念进步的背景下教授的。这个 课程邀请在各自领域做出重大贡献的讲师提供最新信息 关于当前研究的报告。受训人员是由课程教员从更大的 申请者范围从研究生到高级调查人员和研究医生。因为 在这一课程的短期内，高年级和低年级学生都可以参加并获得短期的、 在远离其他对时间和注意力的要求的环境中进行高强度的训练。 该课程还为知名科学家提供了一个难得的机会来应用这些先进的 技术，以满足他们自己的研究兴趣，或在神经科学的重要专业进行再培训 他们可能并不熟悉。课程中教授的方法和概念被传播给 通过出版实验室手册和在线资源，向更广泛的研究界宣传。

ABSTRACT CSHL Laboratory Course in Single Cell Analysis The proposed Cold Spring Harbor Laboratory (CSHL) course Single Cell Analysis to be held annually in July 2023– 2027, is part of the CSHL’s postgraduate training program in cell and molecular biology. This is a short, intensive 2 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of singe cell-based research. General themes to be covered during the course include quantitative single cell analysis by RNAseq, genomic DNA analysis, proteomics, and metabolomics. Specific experimental techniques taught in the course include: 1) Droplet- and microwell based single cell isolation; 2) Single cell RNA seq library preparation; 3) Single molecule FISH; 4) Single cell peptidomics; 5) Single cell western blot; 6) Photoactivatable single cell probes; 7) Single cell mass spectrometry; 8) Soft X-ray tomography; 9) Introductory single cell sequencing analysis. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.