Tissue Pathology and Biospecimen Shared Resource

组织病理学和生物标本共享资源：摘要 组织病理学和生物标本共享资源（TPB SR）提供组织学服务和采购， 库，并分发人类癌症和控制组织和液体样本。城市规划委员会的特别报告员是Kar- Ming Fung，MD，PhD（CT），病理学家，同时也是SCC研究人员和其他研究人员之间的联络人。 病理学家在报告期内，城规会策略检讨成功整合了原有的 将SCC生物标本储存库纳入TPB SR，并提供新建立的注释数据库和在线 catalog.其他成就包括一个新的NIH/OD S10赠款和三个地方赠款， 基于荧光的全载玻片扫描与图像分析，并扩大自动化的能力， 原位杂交为了确保质量和专业化，城市规划委员会特别报告员分为两个部门。组织 病理学组提供：1）实验设计咨询和组织病理学协助 解释; 2）从组织处理，包埋和切片的完整组织学服务 组织/细胞块微阵列的构建; 3）自动化单或多路 免疫组化、免疫荧光、原位杂交和TUNEL，使用色原或 4）利用图像分析的明场和荧光全载玻片扫描;以及 5)循环肿瘤细胞的分离和激光捕获显微术。生物标本臂提供：1） 新鲜标本和固定标本的采购和银行服务，并附有注释数据库; 2）在线 机构内联网上的收藏目录，带有仪表板类型的显示;以及3）搜索和检索 病理学系的档案材料网站和数据以电子方式存储， 物理隔离的计算机，以防止信息泄露。两个组都被安置在SCC指定的 在OUHSC的实验室研究综合体（BRC & BMSB）的空间，一个方便的位置，为大多数（52 共63名）实验室SCC成员。从2017年到2021年，癌症生物学（CB），癌症治疗学的使用 (CT)癌症预防与控制（CPC）计划成员的比例分别为58%、36%和6%。 在此期间，SCC成员（正式成员和准成员）占SR总使用量的60%。SCC成员 同行评审支持占总SR使用量的一半以上（52%），87%的SCC成员 使用. SCC附属成员（即，从事癌症相关工作的非CCSG成员）占 占总使用量的20%。TPB SR生成的数据用于确保11项同行评审 癌症研究赠款以及许多其他赠款和科学出版物。城规会提供的SR服务 免费提供给SCC会员。这种费用豁免每年由SCC董事批准， 为SCC成员促进研究提供了显著的增值效益。

TISSUE PATHOLOGY AND BIOSPECIMEN SHARED RESOURCE: ABSTRACT The Tissue Pathology and Biospecimen Shared Resource (TPB SR) provides histology services and procures, banks, and distributes human cancer and control tissue and fluid samples. The TPB SR is directed by Kar- Ming Fung, MD, PhD (CT), a pathologist who also serves as a liaison between SCC researchers and other pathologists. During the reporting period, the TPB SR completed a successful integration of the pre-existing SCC biospecimen repository into the TPB SR with a newly established annotated database and an online catalog. Other accomplishments include a new NIH/OD S10 grant and three local grants to establish fluorescence-based whole-slide scanning with image analysis and to expand the capabilities in automated in situ hybridization. To ensure quality and specialization, the TPB SR is organized into two arms. The tissue pathology arm provides: 1) consultation on experimental design and assistance in histopathologic interpretation; 2) a complete line of histology services from tissue processing, embedding, and sectioning paraffin and frozen bocks to construction of tissue / cell block microarrays; 3) automated single or multiplex immunohistochemistry, immunofluorescence, in situ hybridization and TUNEL using chromogen- or fluorescence-based detection; 4) bright-field and fluorescence whole-slide scanning with image analysis; and 5) isolation of circulating tumor cells and laser capture microscopy. The biospecimen arm provides: 1) procurement and banking services for fresh and fixed specimens with an annotated database; 2) an online collection catalog on the institutional intranet with a dash-board type display; and 3) search and retrieval of archival materials from the Department of Pathology. The website and data are stored in electronically and physically separated computers to prevent breach of information. Both arms are housed in SCC-designated space in the OUHSC’s laboratory research complex (BRC & BMSB), a convenient location for a majority (52 of 63 total) lab-based SCC members. From 2017 to 2021, usage by Cancer Biology (CB), Cancer Therapeutics (CT), and Cancer Prevention and Control (CPC) program members was 58%, 36% and 6%, respectively. During this period, SCC members (full and associate) accounted for 60% of total SR usage. SCC members with peer-reviewed support accounted for more than half (52%) of total SR usage, and 87% of SCC member usage. SCC affiliate members (i.e., non-CCSG members who conduct cancer-related work) accounted for another 20% of total usage. Data generated by the TPB SR was used to secure 11 peer-reviewed funded cancer research grants as well as many other grants and scientific publications. TPB SR services are offered free-of-charge to SCC members. This waiver of fees is approved on an annual basis by the SCC director and provides a significant value-added benefit for SCC members to foster research.