Stable Isotope Analytical Core for Studies in Human Metabolism

描述(由申请人提供)：稳定同位素示踪方法能够在体内量化代谢途径的急性反应。在以前的许多研究中，我们已经表明，稳定同位素代谢研究的结果准确地预测了营养治疗的长期结果。这一核心的一个主要目标将是支持整个科学界利用稳定同位素方法学进行的代谢研究。对稳定同位素示踪剂研究的支持将包括样本和数据分析以及培训。我们还将根据需要继续开发新的方法来支持这些研究。此外，我们将积极培训研究人员稳定同位素示踪方法学。最后，我们将寻求在世界各地扩大合作，为我们的分析服务开发一个市场，并提供持续的财务支持。该核心将优先为NIH资助的项目进行稳定同位素分析；然而，任何使用稳定同位素方法的调查人员都可以要求分析服务。PI是一位在稳定同位素方法学方面经验丰富且备受尊敬的研究员，他从UTMB和Shriners转到阿肯色大学，并得到了大学的极好支持。该项目的目的是为使用稳定同位素方法的研究补助金提供分析支助；开发新的方法来支持新陈代谢过程的研究；教育科学界关于在人体内使用稳定同位素的示踪剂方法的应用；并加强合作以发展客户，以获得持续的资金支持。 项目简介：这项提议描述了一个稳定的同位素分析核心，以支持世界范围内的人类代谢研究。核心以最低的服务费提供常规和专门的样本分析。此外，它还将在人类代谢研究中使用稳定同位素的方法开发和培训方面提供援助。

DESCRIPTION (provided by applicant): Stable isotope tracer methodology enables in vivo quantification of acute responses of metabolic pathways. In many previous studies we have shown that the results of metabolic studies with stable isotopes have accurately predicted long-term outcomes of nutritional therapies. A primary goal of this core will be to support metabolic research utilizing stable isotope methodology throughout the scientific community. Support of stable isotope tracer studies will include sample and data analysis, and training. We will also continue to develop new methodologies as needed to support these studies. In addition, we will actively train investigators on stable isotope tracer methodology. Finally, we will seek to extend collaborations throughout the world to develop a market for our analytical services and to provide for continued financial support. The core will prioritize stable isotope analyses for NIH-funded projects; however, any investigator utilizing stable isotope methodology may request analytical services. The PI is an experienced and well respected researcher in stable isotope methodology who moved from the UTMB and Shriners to University Arkansas and is receiving excellent support from the university. The aims of this project are to provide analytical support for research grants utilizing stable isotope methodology; to develop new methods to support studies of metabolic processes; to educate the scientific community regarding the in vivo applications of tracer methodology using stable isotopes in human subjects; and enhance collaborations to develop a clientele for continued financial support. Project Narrative: This proposal describes a stable isotope analytical core to support world-wide research in human metabolic studies. The core offers routine and specialized sample analysis on a minimal fee-for-service basis. In addition, it will provide assistance in methods development and training in the use of stable isotopes in human metabolic studies.