BD Biosciences LSR II FACS for the University of South Carolina

描述（由申请人提供）：该提案要求277，596美元购买BD Biosciences LSR II FACS系统。该系统将安置在南卡罗来纳州大学医学院和结肠癌研究中心的仪器资源设施（IRF）中。国际研究基金会是一个已建立的核心生物技术设施，为南卡罗来纳州大学以及南卡罗来纳州的其他几个本科和研究生院提供服务。南卡罗来纳州大学唯一可用的核心设施FACS系统是15年前的Beckman Coulter Epics XL，其在激光、荧光染料激发和发射、信号检测和数据分析方面非常过时。值得注意的是，该提案的两个主要用户是大型中心赠款的PI，一个是结肠癌，一个是自身免疫性疾病和炎症。该系统的主要用户将是7名NIH资助的研究人员以及一名初级研究员，Udai Singh博士，他是IRF的研究助理教授。除了两个中心赠款，该小组还担任11个R 01赠款的PI和其他一些NIH赠款的Co-Is。该系统将用于各种研究领域，包括炎症，免疫学，癌症，艾滋病毒/艾滋病和心血管发育和疾病。正如提案中所详述的那样，南方大学的生物医学研究环境正在迅速扩大，研究界目前还没有能够检测四种以上颜色的最先进的流式细胞仪系统。这对参与这项提案的研究人员的研究计划以及南卡罗来纳州大学招募其他有前途的科学家产生了不利影响。收购该系统并将其安置在已建立的核心设施中，将允许大量研究人员使用它，并将显着改善南卡罗来纳州和南卡罗来纳州的研究环境。公共卫生相关性：细胞分选在许多涉及炎症的疾病如癌症、HIV和心血管肥大的研究中是必不可少的。存在的多种细胞类型的免疫学和区分存在的细胞类型及其各种生物标志物的能力最容易用高分辨率、多色、高通量仪器如所要求的BD Biosciences LSR II分选仪进行。

DESCRIPTION (provided by applicant): This proposal requests $277,596 to purchase a BD Biosciences LSR II FACS system. The system will be housed in the Instrumentation Resource Facility (IRF) of the University of South Carolina School of Medicine and Center for Colon Cancer Research. The IRF is an established core biotechnology facility serving the University of South Carolina as well as several other undergraduate and graduate institutions in the State of South Carolina. The only core facility FACS system available at the University of South Carolina is a 15 year old Beckman Coulter Epics XL, which is very outdated with regard to lasers, fluorochrome excitation and emission, signal detection, and data analysis. Notably, two of the Major Users on this proposal are PIs of large Center grants, one on colon cancer and one on autoimmune diseases and inflammation. Major users of the system will be 7 NIH funded researchers as well as a Junior Investigator, Dr. Udai Singh who is a Research Assistant Professor in the IRF. In addition to the two Center Grants the group serves as PIs of 11 R01 grants and Co-Is on a number of other NIH grants. The system will be used in a variety of research areas including inflammation, immunology, cancer, HIV-AIDS and cardiovascular development and disease. As detailed in the proposal the biomedical research environment at the University of South is rapidly expanding and no state of the art FACS systems capable of detecting more than four colors is available to the research community. This has adversely affected the research programs of the investigators participating in this proposal as well as the recruitment to the University of South Carolina of other promising scientists. Acquisition of this system and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research environment at the University of South Carolina and in the State of South Carolina. Public Health Relevance: Cell sorting is essential in the study of many diseases that involve inflammation such as cancer, HIV, and cardiovascular hypertrophy. The immunology of the multiple cell types present and the ability to distinguish the types of cells present and their various biomarkers is most readily performed with high resolution, multi-color, high throughput instrumentation such as the requested BD Biosciences LSR II sorter.