Request for Funds to Purchase a Replacement Laser Scanning Confocal Microscope

描述（由申请人提供）：蔡司LSM 710激光扫描共聚焦显微镜将成为爱荷华州大学NIH研究人员的重要工具。拟议中的显微镜将取代一个失败的系统，在过去的18个月里，该系统支持了55名爱荷华州国立卫生研究院资助的研究人员的共聚焦成像需求，目前的增量总成本为86，075，859美元。新系统将位于Eckstein医学研究大楼的1号房间，由爱荷华州大学中央显微镜研究设施（CMRF）监督。管理、维护以及高级技术援助和培训将由经验丰富的工作人员提供。蔡司LSM 710将全天24小时提供，以最好地适应调查人员。研究副总裁Meredith Hay和CMRF主任Kenneth摩尔的支持证明了强有力的机构承诺。本提案中强调的研究人员是NIH资助的生物医学研究的一小部分，蔡司LSM 710将加强这些研究。Joseph Zabner将使用Zeiss LSM 710对与哮喘、气道防御机制、基质金属蛋白酶-9和腺相关病毒囊性纤维化基因治疗相关的样本进行成像。Robert Mullins将研究年龄相关性黄斑变性中内皮细胞激活的病理生理学。凯瑟琳·斯卢卡将研究慢性肌肉疼痛。保罗·麦克雷将研究SARS-冠状病毒感染。吴仲芳从事神经肌肉接头突触终扣形成改变的基础生物学研究。Steven Clegg将对生长在各种生物表面上的肠杆菌生物膜进行成像。约瑟夫巴克沃尔特将研究方法，以防止创伤后骨关节炎的急性影响。这些NIH资助的教职员工将受益于蔡司LSM 710激光扫描显微镜的可靠可用性和先进的新功能。公共卫生相关性：蔡司LSM 710激光扫描共聚焦显微镜将成为爱荷华州大学NIH研究人员的重要工具。拟议中的显微镜将取代一个失败的系统，在过去的18个月里，该系统支持了55名爱荷华州国立卫生研究院资助的研究人员的研究，目前由86,075,859美元的增量总成本支持。本申请中强调的项目代表了多种健康相关研究，这些研究正在发现与人类呼吸道病原体（包括SARS和其他病毒性疾病）、潜在基因转移载体（例如腺相关病毒和猫流感病毒）、气道上皮防御机制相关的新见解。囊性纤维化、黄斑变性、慢性肌肉疼痛、细菌生物膜和创伤后骨关节炎。

DESCRIPTION (provided by applicant): The Zeiss LSM 710 Laser Scanning Confocal Microscope will be a critical tool for NIH investigators at the University of Iowa. The proposed microscope will replace a failing system that over the past 18 months has supported the confocal imaging needs of 55 University of Iowa NIH funded Investigators currently supported by $86,075,859 in incremental total costs. The new system will be located in room 1 of the Eckstein Medical Research Building under the supervision of The University of Iowa Central Microscopy Research Facility (CMRF). Administration, maintenance as well as high-level technical assistance and training will be provided by experienced staff. The Zeiss LSM 710 will be made available 24 hrs a day to best accommodate investigators. Strong institutional commitment is demonstrated by the support of Vice President for Research Meredith Hay and CMRF Director Kenneth Moore. The group of investigators highlighted in this proposal is a small subset of the NIH funded biomedical research that will be enhanced by the Zeiss LSM 710. Joseph Zabner will use the Zeiss LSM710 to image samples related to asthma, airway defense mechanisms, matrixmetalloproteinase-9 and Adeno-Associated virus cystic fibrosis gene therapy. Robert Mullins will investigate the pathophysiology of endothelial cell activation in age-related macular degeneration. Kathleen Sluka will research chronic muscle pain. Paul McCray will study SARS-Coronaviirus infection. Chung-Fang Wu conduct research on the basic biology of altered synaptic bouton formation at neuromuscular junctions. Steven Clegg will image enterobacteria biofilms growing on various biotic surfaces. Joseph Buckwalter will investigate methods to prevent the acute effects of post-traumatic osteoarthritis. These NIH funded faculty members will benefit greatly from the reliable availability and advanced new features the Zeiss LSM 710 Laser Scanning Microscope. PUBLIC HEALTH RELEVANCE: The Zeiss LSM 710 Laser Scanning Confocal Microscope will be a critical tool for NIH investigators at the University of Iowa. The proposed microscope will replace a failing system that over the past 18 months has supported the research of 55 University of Iowa NIH funded Investigators currently supported by $86,075,859 in incremental total costs. The projects highlighted in this application are representative of a diverse group of health-related research that is discovering new insights related to human respiratory pathogens including SARS and other viral diseases, potential gene transfer vectors such as Adeno-Associated Virus and Feline Influenza Virus, airway epithelia defense mechanisms, Cystic Fibrosis, Macular Degeneration, Chronic Muscle Pain, Bacterial Biofilms and Post-Traumatic Osteoarthritis.