ACTIN-DEPENDENT ENDOCYTOSLS IN BUDDING YEAST

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在酵母细胞中，内吞作用完全依赖于肌动蛋白细胞骨架。我们目前研究的一个主要领域是 阐明肌动蛋白的内吞作用。我们已经分离出了许多突变体，这些突变体可以在不连续的步骤中阻断酵母中的内吞作用 在这个过程中，我们用GFP标记了大约20-30种内吞和细胞骨架蛋白。我们会用X射线断层扫描 充分阐明了内吞途径的空间组织，并将该途径与各种其他细胞 功能.我们将确定这些特征如何通过细胞周期变化，以响应内吞诱导的细胞周期。 Ste 2 p受体蛋白的内吞作用及其对特异性内吞蛋白和细胞骨架蛋白突变的响应

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In yeast cells, endocytosis is entirely dependent upon the actin cytoskeleton. A major current area of our research is to elucidate the endocytic roles of actin. We have isolated numerous mutants that block endocytosis in yeast at discrete steps in the process, and we tagged some 20-30 endocytic and cytoskeletal proteins with GFP. We will use x-ray tomography to fully elucidate the spatial organization of the endocytic pathway and to relate this pathway with a variety of other cellular features. We will determine how these features change through the cell cycle in response to endocytic induction of the Ste2p receptor protein endocytosis and in response to mutation of specific endocytic and cytoskeletal proteins