2nd U.S.-Canada Winter School on Biomolecular Solid State NMR

描述（由申请人提供）：在过去的5-10年里，固态核磁共振（SSNMR）已经成为一种技术，可以产生重要的结构数据，这些数据不能用x射线衍射或溶液核磁共振进行检查，目前结构生物学的两大支柱。例如，SSNMR实验最近产生了一些膜肽和蛋白质的第一个结构，以及第一个实验确定的淀粉样蛋白原纤维的结构，因为它们是不溶的，不能在其生物学相关状态下结晶，因此其他技术无法对其进行详细研究。这些最近的成功是光谱和样品制备方法的基本和实际改进的结果。同时，从ssmr发展中出现的技术，例如平均哈密顿理论，影响了其他研究领域，如溶液核磁共振和磁共振成像。最后，最初用于SSNMR实验的动态核极化（DNP）灵敏度增强的复兴在溶液核磁共振和成像中得到了广泛的应用，并进一步将高场电子顺磁共振（EPR）和核磁共振整合到一个单一的歧管中。为了将这些进展迅速传播到科学界，我们建议于2010年1月24日至29日在加州斯托举办第二届固体生物分子核磁共振冬季学校。该学院的主要组成部分将是由该领域的主要研究人员就基础和高级主题进行一系列讲座。主题将包括现代SSNMR数学理论的基本方面，特定的最先进的实验技术的实际优势的评估，同位素标记蛋白质和肽的制备方法，数据处理和数值模拟，从SSNMR数据的结构模型的发展等。讲座将辅以问题集和实际操作演示软件（SPINEVOLUTION， SIMPSON， GAMMA等），目前用于模拟生物固体的重要脉冲核磁共振实验。在这所学校的学习将使研究生和博士后学生接触到广泛的基本技术和概念，极大地提高了他们通过自己的研究小组的特定研究项目获得的更专业的训练。这是第二所SSNMR学校，我们从2008年1月第一所学校的学生和博士后那里收到了非常积极的反馈，我们正在组织这所学校。因此，我们相信冬季学校对于蓬勃发展的固态生物核磁共振社区来说是一个极其重要的教学活动。

DESCRIPTION (provided by applicant): In the past 5-10 years, solid state NMR (SSNMR) has emerged as a technique that yields important structural data on systems not amenable to examination with either X-ray diffraction or solution NMR, presently the two pillars of structural biology. For example, SSNMR experiments have recently produced some of the first structures of membrane peptides and proteins and the first experimentally-determined structures of amyloid fibrils, which have been resistant to detailed investigation by other techniques because they are insoluble and do not crystallize in their biologically relevant state. These recent successes are a result of fundamental and practical improvements in spectroscopic and sample preparation methods. Concurrently, technology emerging from developments in SSNMR, for example Average Hamiltonian Theory has influenced other areas of research such as solution NMR and magnetic resonance imaging. Finally, the renaissance in sensitivity enhancement via dynamic nuclear polarization (DNP) originally intended for SSNMR experiments is finding wide application in solution NMR and imaging and is further integrating high field electron paramagnetic resonance (EPR) and NMR into a single manifold. In order to rapidly propagate these advances to the scientific community we propose to hold the 2nd Winter School on Biomolecular Solid State NMR in Stowe, VT January 24-29, 2010. The principal component of this school will be a series of lectures on fundamental and advanced topics by leading researchers in the field. Topics will include basic aspects of the mathematical theory of modern SSNMR, assessment of practical advantages of specific state-of-the-art experimental techniques, methods for preparation of isotopically labeled proteins and peptides, data processing and numerical simulation, the development of structural models from SSNMR data, etc. The lectures will be supplemented by problem sets and hands-on demonstrations of software (SPINEVOLUTION, SIMPSON, GAMMA, etc.) currently used to simulate pulsed NMR experiments important for biological solids. Attendance at this school will expose graduate and postdoctoral students to a broad spectrum of essential techniques and concepts, greatly enhancing the more specialized training they would otherwise obtain through specific research projects in their own research groups. This is the 2nd SSNMR school and is being organized in response to the very positive feedback that we received from the students and postdoctorals that attended the 1st school in January 2008. As a result we believe the Winter School an extremely important pedagogical event for the blossoming solid state biological NMR community.