High Performance NMR uFlowProbe Optimized for Mass-Sensitive Investigation of Wel

描述（由申请人提供）：在过去五年中，微升体积、高质量灵敏度NMR检测探针的开发产生了重大影响。结合灵敏度、简单性和经济性，这些室温探针需要非常小的初始匀场，并且在各个样品之间几乎不需要匀场或调谐调整，并且不需要低温冷却探针技术的高成本或维护要求，同时提供相当的质量灵敏度。因此，它们非常适合分析液相色谱馏分中典型的低丰度分析物。现在提出的努力提供了扩展该产品的能力，以包括19F检测，由于19F在化学位移方面提供的固有化学分散以及由于其在生物样品中的天然缺失，该技术对大学研究以及制药和其他化学产品行业非常重要。该提案专门寻求资金，以开发一种新的探针，可以完成1H和19F检测（使用单个探针），以获得无背景的高质量数据。该提议的一种有利方法利用Protasis拥有的独特的双流通池架构，以基本上将两个单独优化的探针功能整合到单个机械探针封装中。新一代经济实用的NMR分析仪对人类健康的潜在影响非常大。 公共卫生相关性：该提案寻求资金来开发一种新的质量敏感探针，该探针可以完成1H和19F检测（使用单个探针），以获得来自低丰度分析物的无背景质量数据，例如来自液相色谱的馏分。要求这种新一代高性能NMR检测技术的研究小组包括制药行业，他们需要一种经济合理的方法，使用比过去更少量的样品将19F检测纳入发现和开发工作中，以及除了经济之外还希望实现在较小尺寸规模下工作的性能优势的学术研究人员。新一代经济实用的NMR分析仪对人类健康的潜在影响非常大。

DESCRIPTION (provided by applicant): The impact over the past five years of the development of microliter-volume, highly mass-sensitive NMR detection probes has been substantial. Combining sensitivity, simplicity, and economy, these room-temperature probes require very minor initial shimming and virtually no shimming or tuning adjustments between individual samples, and do not carry the high cost or maintenance requirements of cryogenically-cooled probe technologies while providing comparable mass sensitivity. As such, they are ideally suited to the analysis of low-abundance analytes typical of liquid chromatography fractions. The proposed effort now offers to extend this product capability to include 19F detection, a technique that is of great importance to university research and to pharmaceutical and other chemical product industries due to the inherent chemical dispersion that 19F offers in terms of chemical shift and due to its natural absence in biological samples. This proposal specifically seeks funding to develop a new category of probe that can accomplish both 1H and 19F detection (using a single probe) to obtain background-free, quality data. A favored approach of this proposal utilizes a unique dual flowcell architecture owned by Protasis to essentially incorporate two separately-optimized probe functionalities into a single mechanical probe package. The potential impact on human health resulting from new generations of economical and practical NMR-based analyzers is extremely high. PUBLIC HEALTH RELEVANCE: This proposal seeks funding to develop a new category of mass-sensitive probe that can accomplish both 1H and 19F detection (using a single probe) to obtain background-free, quality data from low-abundance analytes, e.g. fractions originating from liquid chromatography. Research groups asking for this new generation of high performance NMR detection technology include the pharmaceutical industry, who require an economically-justifiable means to include 19F detection in discovery and development efforts using smaller amounts of sample than was available in the past, and academic researchers who in addition to economy also wish to realize the performance advantages of working at smaller size scale. The potential impact on human health resulting from new generations of economical and practical NMR-based analyzers is extremely high.