New Targets for Reproductive Control of Mosquito Vectors

描述(申请人提供)：蚊媒繁殖控制的新目标。在这个项目中，我们将研究登革热和基孔肯雅病毒的两种主要疾病媒介：黄热病蚊子(埃及伊蚊)和亚洲虎蚊子(白纹伊蚊)的雄性精液蛋白(SFP)。登革热和基孔肯雅热在全球的发病率都在增加，迫切需要为这两个物种制定新的和有效的病媒控制战略。在这项研究中，我们将进行两个具体的目标，以确定和功能特征，首次在Ae。Aegypti和Ae.白纹伊蚊。我们的目标是直接识别新的蚊子SFP靶标，这些靶标可以被操纵为一种新的生殖控制手段，或者修改传播致命病原体的昆虫的血液生理，长期目标是减少或消除病毒对人类的传播。 公共卫生相关性：在这项研究中，我们将直接鉴定和确定雄性蚊子产生的精液蛋白的功能，并在交配过程中转移到雌性蚊子身上。我们将确定可以被操纵的目标，作为一种新的生殖控制手段，或作为一种改变昆虫觅血生理的手段，这些昆虫传播导致登革热和基孔肯雅热等疾病的致命病原体，长期目标是减少或消除病毒对人类的传播。

DESCRIPTION (provided by applicant): New Targets for Reproductive Control of Mosquito Vectors. In this project, we will investigate the male seminal fluid proteins (SFPs) of two major disease vectors of dengue and Chikungunya viruses: the yellow fever mosquito (Aedes aegypti) and the Asian tiger mosquito (Aedes albopictus). Both dengue and Chikungunya are increasing in incidence globally and there is an immediate need to develop novel and effective vector control strategies for these two species. In this study we will conduct two specific aims to identify and functionally characterize SFPs, for the first time, in Ae. aegypti and Ae. albopictus. Our goal is to directly identify new mosquito SFP targets that could be manipulated as a novel means of reproductive control, or to modify the blood feeding physiology of insects that transmit deadly pathogens, with the long-term goal to reduce or eliminate virus transmission to people. PUBLIC HEALTH RELEVANCE: In this study, we will directly identify and determine the function of seminal fluid proteins produced in male mosquitoes and transferred to females during mating. We will identify targets that could be manipulated as a novel means of reproductive control or as a means of modifying the blood feeding physiology of insects that transmit deadly pathogens causing diseases such as dengue and Chikungunya, with the long-term goal to reduce or eliminate virus transmission to people.