15 Tesla Bruker solariX FTICR MS

描述（由申请人提供）：俄亥俄州州立大学拟购买Bruker solariX 15特斯拉傅里叶变换离子回旋共振质谱仪（FTICR MS）。这种多功能的高端仪器将被放置在校园化学仪器中心（CCIC）内的OSU质谱和蛋白质组学设施（MS&P）中，CCIC是一个校园范围的中心，行政上位于OSU研究办公室。该仪器将由质谱和蛋白质组学设施的工作人员以及从质谱和主要用户研究小组中挑选的训练有素的人员操作。该仪器的主要吸引人的特点是它的应用范围，与双MALDI和ESI源耦合到高分辨率和高质量精度。拟议的FTICR MS提供高达40，000，000的分辨率，亚ppm的质量精度和低至100阿莫尔范围的灵敏度。在外部碰撞室中通过碰撞诱导解离和电子转移解离（ETD）进行的碎片化以及在ICR室中通过持续的非共振照射（SORI）和电子转移解离是可能的，并且提供了互补的碎片化信息。CASI（连续积累的选定离子）是可用于丰富非常低丰度的物种，通过捕获在碰撞池，甚至从组织成像。已经确定了一个主要的用户群体，他们已经使用质谱法来解决生物医学问题，但由于无法获得更高性能的仪器（如solariX），他们的研究受到限制。这些包括来自医学院（Torrelles，Rovin，Freitas，Parthun），综合癌症中心（克林顿），化学和生物化学（Schweiler，Foster，Musier-Forsyth和Turro）和物理学（Poirier和Zhong）的教师。这些研究人员将解决的问题类型，以及他们将与所提出的solariX FTICR MS一起使用的技术，包括（1）组织的高分辨率MALDI和MALDI成像，（2）详细的高端，特定组蛋白和后修饰蛋白质的自上而下的结构研究，（3）天然质谱测量以获得蛋白质复合物的化学计量和拓扑结构，以及（4）用于代谢物和金属-DNA络合物的结构表征的络合物混合物分析。CCIC MS&P设施是一个建立，利用良好的大学范围内的核心。它为高端仪器提供了一个最佳的环境-一个充满活力的研究社区，非常需要这样的仪器和俄勒冈州立大学的教职员工，他们有热情和专业知识来利用solariX来发挥其最大的潜力。如果得到资助，solariX将对非常广泛的研究人员群体产生不可估量的影响。

DESCRIPTION (provided by applicant): The Ohio State University proposes to acquire a Bruker solariX 15 tesla Fourier Transform Ion Cyclotron Resonance mass spectrometer (FTICR MS). This versatile high-end instrument will be placed in the OSU Mass Spectrometry and Proteomics Facility (MS&P) within the Campus Chemical Instrument Center (CCIC), a campus-wide Center that is administratively housed in the OSU Office of Research. The instrument will be operated by staff of the MS and proteomics facility and selected highly trained personnel from mass spectrometry and major user research groups. This instrument's main attractive features are its breadth of application, with a dual MALDI and ESI source coupled to ultrahigh resolution and high mass accuracy. The proposed FTICR MS provides resolution of up to 40,000,000, sub ppm mass accuracy, and sensitivity down to the 100 amol range. Fragmentation by collision-induced dissociation and electron transfer dissociation (ETD), in the external collision cell, and sustained off-resonance irradiation (SORI) and electron transfer dissociation in the ICR cell are possible and provide complementary fragmentation information. CASI (Continuous Accumulation of Selected Ions) is available to enrich very low abundant species by trapping in the collision cell, even from tissue imaging. A major user group that already uses mass spectrometry to solve biomedical problems, but that have faced limitations in their research because they do not have access to a higher performance instrument such as the solariX, has been identified. These include faculty from the College of Medicine (Torrelles, Rovin, Freitas, Parthun), the Comprehensive Cancer Center (Clinton), Chemistry and Biochemistry (Bruschweiler, Foster, Musier-Forsyth, and Turro), and Physics (Poirier and Zhong). The types of problems that will be solved for these investigators, and techniques that they will use with the proposed solariX FTICR MS, include (1) high resolution MALDI and MALDI imaging of tissue, (2) detailed high end, top down structural studies of specific histone proteins and post-translationally-modified proteins, (3) native mass spectrometry measurements to gain stoichiometry and topology of protein complexes, and (4) complex mixture analysis for structural characterization of metabolites and metal-DNA complexes. The CCIC MS&P Facility is an established, well utilized university-wide core. It provides an optimum environment for a high end instrument - a vigorous research community with great need for such an instrument and OSU faculty and staff with the enthusiasm and expertise to use the solariX to its greatest potential. If funded, the solariX will be invaluable to a very broad constituency of researchers.