Gradient thermal cycler

概述所申请的设备（Eppendorf Mastercycler ep Gradient Thermal Cycler）将支持各种研究项目，这些项目可分为三个主要领域：基因组学、种群遗传学和保护。1）种群遗传学：所申请的设备将用于研究重组在有性和无性谱系长期进化中的作用。这将提供高通量的DNA扩增能力，筛选有性和无性生殖水蚤谱系的全基因组遗传多态性所必需的。 2)基因组学：比较连锁图谱可以为染色体进化、基因组重组和物种形成提供意想不到的见解，并可以揭示基因组间重组率变化的性质和规模。 所要求的设备将用于调查水蚤属密切相关物种之间的重组率变化。3）保护：分子方法在保护生物学中的应用彻底改变了濒危物种和生态系统的管理。所要求的设备将在查明水生物种入侵途径方面发挥关键作用（例如栉水母拟记忆虫和五种被囊动物），这些设备将支持现有的研究方案和及时完成研究生研究。

SummaryThe requested equipment (the Eppendorf Mastercycler ep Gradient Thermal Cycler) will support diverse research projects which can be categorized into three main areas: genomics, population genetics and conservation.1) Population genetics:  The requested equipment will be used for studying the role of recombination in the long term evolution of sexual and asexual lineages. It will provide high through-put DNA amplification capacity necessary for screening the genome-wide genetic polymorphism in sexual and asexual Daphnia lineages.  2) Genomics:  Comparative linkage mapping can provide unexpected insights into chromosome evolution, genome reorganization and speciation and can reveal the nature and scale of recombination rate variation across genomes.  The requested equipment will be used for investigating recombination rate variation among closely related species of Daphnia.3) Conservation:  The application of molecular approach to conservation biology has revolutionized the management of endangered species and ecosystems. The requested equipment will play a key role in the identification of routes of aquatic species invasions (e.g. ctenophore Mnemiopsis, plus five species of tunicates).This equipment will support existing research programs and timely completion of graduate research.