The role of the MYST family transcriptional co-activator, Mof, in embryonic development

生物学的一项主要任务是了解人类基因组如何指导单个细胞的发育形成完整的个体。显然，这项任务的很大一部分是了解基因的表达在胚胎发育过程中是如何调节的。基因表达需要辅激活复合物。辅激活因子复合物通常含有调节染色质（DNA和组蛋白的复合物）结构的蛋白质。然而，大多数共激活剂的生理功能是完全不清楚的。本项目的目的是研究Mof在胚胎发育过程中的功能。Mof是一种通过修饰组蛋白直接调节染色质结构的共激活剂。Mof是MYST家族的一员，包括Moz和Qkf。我们最近表明，Moz和Qkf是必不可少的造血干细胞群和神经干细胞群，分别。本项目的目的是对Mof在体内的功能进行详细的分析，并确定其在调节基因表达中的重要性。所有生物过程都依赖于基因转录的精确调节，所有疾病，无论是涉及病原体还是细胞内在病理变化，如癌症，都会导致基因表达的变化。染色质结构的调节已被确定为健康和疾病中转录调节的主要机制。然而，我们对体内调控染色质结构的精确分子机制的理解非常有限。这项工作将充分调查的作用，一个重要的辅激活剂在体内，包括机制分析。这将增加对基因表达如何调节的理解，最终，这些知识将在新治疗模式的开发中得到广泛应用。

A major task in biology is to understand how the human genome directs the development of a single cell to form an entire individual. Clearly, a large part of this task is to understand how the expression of genes is regulated during embryonic development. Gene expression requires co-activator complexes. Co-activator complexes typically contain proteins which regulate the structure of chromatin (a complex of DNA and histones). However, the physiological function of most co-activators is entirely unclear. The aim of this project is to study the function of Mof during embryonic development. Mof is a co-activator that directly regulates chromatin structure by modifying histones. Mof is a member of the MYST family of co activators, which includes Moz and Qkf. We have recently shown that Moz and Qkf are essential for the haematopoietic stem cell population and the neural stem cell population, respectively. The purpose of this project is to produce a detailed analysis of the function of Mof in vivo and determine it's importance in regulating gene expression. All biological processes relay on accurate regulation of gene transcription and all diseases, whether they involve pathogens or cell intrinsic pathological changes, such as cancer, lead to changes in gene expression. Regulation of chromatin structure has been identified as a major mechanism of transcriptional regulation in health and disease. However, our understanding of the precise molecular mechanisms regulating chromatin structure in vivo are very limited. This work will fully investigate the role of an important co-activator in vivo including a mechanistic analysis. This will increase understanding of how gene expression is regulated and, ultimately, this knowledge will find wide application in the development of new treatment paradigms.