MALDI-TOF mass spectrometer for biomolecule characterization

该提案的目的是获得基质辅助激光解吸电离（MALDI）质谱，用于化学，物理，化学和生物工程以及生物化学，微生物学和免疫学部门的生物大分子表征。 基于质谱的生物分子表征是越来越多的跨学科研究计划所需的关键实验技术，包括生物分析化学，化学生物学，生物材料和生物技术。 所要求的MALDI质谱仪是一种稳健的多功能线性飞行时间（TOF）系统，可用于测定高达500，000道尔顿的大分子的质荷比。除了对下文所述的具体研究项目产生重要影响外，收购该MALDI-TOF系统将加强渥太华大学不同生物分子科学社区的跨学科研究和培训工作。

The aim of this proposal is to acquire matrix assisted laser desorption ionization (MALDI) mass spectrometry for biological macromolecule characterization in the departments of Chemistry, Physics, Chemical and Biological Engineering, and Biochemistry, Microbiology and Immunology. Mass spectrometry based characterization of biomolecules is a key experimental technique required for a growing number of interdisciplinary research programs, including those in bioanalytical chemistry, chemical biology, biomaterials and biotechnology. The requested MALDI mass spectrometer is a robust, versatile linear time-of-flight (TOF) system that can be used to determine mass to charage ratios for macromolecules up to 500,000 Daltons. Beyond its important impact on the specific research programs described below, acquisition of this MALDI-TOF system will enhance interdisciplinary research and training efforts in the diverse biomolecular sciences community at the University of Ottawa.