LC-MS for the Analysis of Small Molecules

我们正在寻求资金，以获得LC-MS系统的质谱仪组件，用于小分子分析。具体来说，我们要求资金购买安捷伦单四轴ES探测器。我们将通过另一来源为配套的1260无限UHPLC系统获得资金。内部LC-MS设备的可用性将支持七个申请人的研究项目。其中四家（伯纳德、Huner和grics）将使用该系统进行植物激素分析。这些分子的丰度非常低，但对整体代谢（特别是对压力的反应）有重大影响。LC-MS是最灵敏、最合适的植物激素分析工具。其他申请人将使用该系统分析与植物对金属胁迫（Macfie）、细菌hopanoids （Valvano）和铁载体（Heinrichs）有关的根分泌物。收集的数据将解决植物和细菌胁迫生物学的基本问题。

We are seeking funds to acquire the mass spectrometer component of an LC-MS system to be used for small molecule analysis. Specifically, we are asking for funds to purchase an Agilent Single Quad ES detector. We will acquire funds through an alternative source for the companion 1260 Infinity UHPLC system. The availability of in-house LC-MS facilities will support the research programs of seven applicants. Four of these (Bernards, Huner and the Grbics) will use the system for plant hormone analysis. These molecules are present in very low abundance, yet have significant impact on overall metabolism (especially in response to stress). LC-MS is the most sensitive and appropriate tool for plant hormone anlysis. Other applicants will use the system for the analysis of root exudates involved in plant response to metal stress (Macfie), bacterial hopanoids (Valvano) and siderophores (Heinrichs). The data collected will address fundamental questions of plant and bacterial stress biology.