Hormonal control of reproduction and growth in fish

多年来，我的长期目标一直是阐明激素对鱼类繁殖和生长的控制。本文的重点是研究一些关键因素对生殖和生长的控制，包括促性腺激素释放激素（GnRH）、性腺激素类固醇、促性腺激素抑制激素（GnIH）（属于rfamily相关肽家族）和甲状腺激素。我的实验室最近的发现为金鱼的雌激素和甲状腺受体亚型结构和功能提供了重要的信息。我们的实验结果也为GnIH对垂体促性腺激素（GTH）和生长激素（GH）产生的季节性影响以及甲状腺激素在生殖调节中的作用提供了重要信息。拟议的研究将继续正在进行的工作，并以鱼类作为合适的实验模型，开展关于垂体促性腺激素和生长激素生产和性腺发育综合控制的新研究。主要假设是下丘脑神经激素、GnRH和GnIH通过控制垂体LH、FSH和GH的产生（目的1）与甲状腺激素（目的2）协同作用，控制促性腺和促生长轴，调节生长发育和分化。拟议的研究将使用系统方法，使用先进的转录组学和代谢组学方法来理解调节生殖和生长的复杂生物过程。本研究将以金鱼和斑马鱼为实验模型，为GnIH、GnRH和甲状腺激素的作用机制提供新的信息。建议的研究将为分子比较内分泌学领域的学生提供高质量的培训。在我的实验室里，多学科的研究保证了学生们在他们自己的研究领域和狭窄的专业领域之外，都能接触到一个激发智力的环境、广泛的技术和合适的学习环境。

My long-term objective over many years has been to elucidate hormonal control of reproduction and growth in fish. The focus here is to investigate the control of reproduction and growth by a number of key factors, including gonadotropin releasing hormone (GnRH), gonadal steroids, gonadotropin-inhibitory hormone (GnIH) that belongs to RFamide-related family of peptides, and thyroid hormones. Recent findings in my laboratory provided critical information on estrogen and thyroid receptor subtypes structure and function in goldfish. Our experimental results also provide important information concerning seasonal effects of GnIH on pituitary gonadotropin (GTH) and growth hormone (GH) production, and the role of thyroid hormones in the regulation of reproduction. The proposed research will continue the work in progress and initiate new lines of inquiry concerning integrated control of pituitary GTH and GH production and gonadal development, using fish as suitable experimental model. The main hypothesis to be tested is that hypothalamic neurohormones, GnRH and GnIH by controlling pituitary LH, FSH & GH production (Objective 1) work in an integrated manner with thyroid hormones (Objective 2) to control gonadotropic and somatotropic axis, and regulate growth development and differentiation. The proposed study will use a system approach, using advanced transcriptome and metabolomic approach to understand complex biological processes that regulate reproduction and growth. The proposed study will provide novel information on the mechanisms of action of GnIH, GnRH and thyroid hormones, using goldfish and zebrafish as experimental model. The proposed research will provide a suitable vehicle for high quality training of students in the areas of molecular comparative endocrinology. The multi-disciplinary research in my lab assures that students are exposed to an intellectually stimulating environment, a wide range of techniques, and a suitable learning environment in their own area of research as well as outside their narrow areas of specializations.